| 存储条件 |
|---|
| Product Name | Anti-NLRP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB:1:1000-1:2000 ICC/IF:1:100-1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NLRP3 |
|---|---|
| Gene Synonyms | AGTAVPRL AII AII/AVP AVP C1orf7 CIAS1 CLR1.1 DFNA34 FCAS FCAS1 FCU KEFH Mmig1 MWS NALP3 PYPAF1 |
| Gene Full Name | NLR family pyrin domain containing 3 |
| Gene Summary | This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Aug 2020] |
| Alternative Names | caterpiller protein 1.1 cold auto-inflammatory syndrome 1 PYPAF1 NALP3 C1ORF7 cold autoinflammatory syndrome 1 homolog cold autoinflammatory syndrome 1 protein cold autoinflammatory syndrome 1 protein homolog cold-induced autoinflammatory syndrome 1 protein cryopyrin cryopyrin NACHT LRR and PYD domains - containing protein 3 deafness autosomal dominant 34 FLJ95925 mast cell maturation-associated-inducible protein 1 member of the NALP family of inflammatory mediators MMIG-1 NACHT domain- leucine-rich repeat- and PYD-containing protein 3 NACHT LRR and PYD containing protein 3 NACHT/LRR/pyrin domain-containing protein 3 nucleotide-binding oligomerization domain leucine rich repeat and pyrin domain containing 3 PYD NACHT and LRR containing protein PYRIN-containing APAF1-like protein 1 |
| Molecular Weight(MW) | 118 kDa |
| Cellular Localization | Cytoplasm; Endoplasmic reticulum; Golgi apparatus; Inflammasome; Membrane; Nucleus; Secreted |
| SwissProt ID | Q96P20 |
|---|
WB
Western blot analysis using NLRP3 antibody against Nih3T3(1) cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ICC/IF
Immunofluorescence analysis of Nih3T3 cells using NLRP3 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
WB
(C-E) The protein levels of ERα, NLRP3, ASC and Casp-1p20 in NCM-460 cells following different drug treatments.
WB
F) The protein levels of ERα and NLRP3 in colon tissues.
WB
(B) The protein level of ERα and NLRP3 in ERα-overexpressing cells
WB
(B) Co-immunoprecipitations of ERα and NLRP3/pro-casp-1/ASC complexes.
WB
(E-F) Western blot results of NLRP3, ER, Wnt2b, and β-catenin different cells.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
抱歉，暂无相关文献
