| 存储条件 |
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| Product Name | Phospho-Smad2 (Ser465+Ser467) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human SMAD2(Accession Q15796), corresponding to amino acid residues around phosphorylated Ser467. |
| Modification | p-Ser465+Ser467 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 ICC/IF:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD2(4087) |
|---|---|
| Gene Synonyms | Drosophila homolog of MADR2 hMAD-2 HsMAD2 JV18 JV18-1 JV181 MAD MAD homolog 2 MAD Related Protein 2 Mad-related protein 2 MADH2 MADR2 MGC22139 MGC34440 Mother against DPP homolog 2 Mothers against decapentaplegic homolog 2 Mothers against |
| Gene Full Name | SMAD2 SMAD family member 2 [ Homo sapiens (human) ] |
| Gene Summary | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012] |
| Target | Phospho-Smad2 (Ser465+Ser467) Antibody detects endogenous levels of Smad2 only when phosphorylated at Ser465+Ser467. |
| Molecular Weight(MW) | 58kDa; 52kD； |
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Overexpression of SMS1 regulates TGF-β1-induced EMT via TGF-β type I receptors. (A)-expression of TβRI and (B)-phosphorylation of Smad2 and Smad2 were measured by western blotting in MDA-MB-231 cells.
IHC
Non-thermal plasma down-regulated the expression of p-Smad 2/3. **p < 0.01. (A) Typical images of p-Smad 2 and p-Smad 3 after immunohistochemical staining in the control and NTP groups. The image in the lower left corner is a magnified view of the blue square. Bar = 200 μm/20 μm. (B) Statistical analysis of p-Smad2/3 on day 21 in the control and the NTP treated wounds.
ICC/IF
ALG3 enhances radioresistance via regulation of TGFBR2 glycosylation. (b) Representative immunofluorescence images of TGFBR2 expression level in cytoplasmic and membrane fractions. (e) Representative immunofluorescence images of p-SMAD2 expression level in cytoplasmic and nuclear fractions. Nuclear translocation of p-SMAD2 was significantly decreased in ALG3-sg and tunicamycin treatment groups.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 ICC/IF:1:50-1:200 |
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| Form | Liquid |
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| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
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