| 存储条件 |
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| Product Name | Anti-HSP70/HSPA4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIF-PIHCWB |
| WB:1:1000-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500 IF-P:1:200-1:2000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HSPA4 |
|---|---|
| Gene Synonyms | 70kDa 87A APG-2 Bb CELE_C12C8.1 CG31359 CG31366 dHSP70 dhsp70Aa Dm-hsp70 Dmel\CG31359 Dmel\CG31366 Dmel_CG31359 Dmel_CG31366 DmHSP70AA DMHSP7D1 HEL-S-103 HEL-S-5a HS24/P52 hs70 Hsc70t HSP 70 Hsp-70 Hsp-70Aa Hsp110 hsp68 HSP70 hsp70 87A hsp70 87C hsp70 Aa hsp70(87A) Hsp70(87C) HSP70-1 HSP70-1A HSP70-1B HSP70-1L HSP70-2 HSP70-3 HSP70-HOM HSP70.1 HSP70.2 Hsp70.3 Hsp70A hsp70A1 HSP70A2 Hsp70A7d hsp70Aa Hsp70B HSP70B1 Hsp70bb Hsp70Bbc Hsp70Bc HSP70I Hsp70RY HSP70T HSP72 HSPA1 HSPA1A HSPA1B Hspa1l HSPA2 HSPA3 HSPH2 Hspt70 Hst70 HSX70 hum70t I79_004332 irp94 mKIAA4025 Msh5 RY |
| Gene Full Name | heat shock protein family A (Hsp70) member 4 |
| Gene Summary | Predicted to enable ATP binding activity. Involved in chaperone-mediated protein complex assembly and protein insertion into mitochondrial outer membrane. Located in cytosol and extracellular exosome. Implicated in Chagas disease. Biomarker of chronic obstructive pulmonary disease; rheumatoid arthritis; type 2 diabetes mellitus; and ulcerative colitis. [provided by Alliance of Genome Resources, Apr 2022] |
| Alternative Names | 68 kDa heat shock protein 70 kda heat shock protein-2 70 kilodalton heat shock protein 87A locus 87C locus ARABIDOPSIS HEAT SHOCK PROTEIN 70 ATHSP70 CG31359-PA CG31366-PA chaperone dnaK-type molecular chaperone HSP70-1 epididymis secretory protein Li 103 epididymis secretory sperm binding protein epididymis secretory sperm binding protein Li 5a heat shock 10kDa protein 1-like heat shock 70 heat shock 70 kD protein 1 heat shock 70 kD protein 2 heat shock 70 kD protein 3 Heat shock 70 kDa protein 1 heat shock 70 kDa protein 1-Hom heat shock 70 kDa protein 1/2 Heat shock 70 kDa protein 1A Heat shock 70 kDa protein 1A/1B Heat shock 70 kDa protein 1B Heat shock 70 kDa protein 1L Heat shock 70 kDa protein 2 heat shock 70 kDa protein 3 Heat shock 70 kDa protein 4 heat shock 70 kDa-like protein 1 heat shock 70-related protein APG-2 heat shock 70kD protein 1A heat shock 70kD protein 1B heat shock 70kD protein 2 heat shock 70kD protein 4 heat shock 70kD protein-like 1 heat shock 70kDa protein 1-like heat shock 70kDa protein 1A heat shock 70kDa protein 1B heat shock 70kDa protein 2 heat shock 70kDa protein 3 heat shock 70kDa protein 4 Heat shock protein heat shock protein 1-like heat shock protein 1A heat shock protein 1B heat shock protein 2 heat shock protein 4 Heat Shock Protein 70 heat shock protein 70-1 Heat shock protein 70-1l heat shock protein 70.1 heat shock protein 70.2 heat shock protein alpha 2 heat shock protein Apg-2 heat shock protein cognate 70 testis heat shock protein family A (Hsp70) member 1 like heat shock protein family A member 1A heat shock protein family A member 1B heat shock protein family A member 1L heat shock protein family A member 2 heat shock protein family A member 4 heat shock protein family H member 2 heat shock protein 110 kDa heat shock protein 70 kDa 1 heat shock protein 70 kDa 2 heat shock protein 70 kDa 3 heat shock protein-70 heat shock-induced protein Heat shock-related 70 kDa protein 2 heat shock-related protein heat shock-related protein hsc70t heat-shock 70-kilodalton protein 1A heat-shock 70-kilodalton protein 1B heat-shock protein 70 HEL S 103 HSP HSP70 1 HSP70 1B HSP70 2 hsp70 87A HSP70 AceyltK77 hsp70 RY HSP70-1/HSP70-2 HSP70.1 HSP70.1/2 HSP70.1/HSP70.2 HSP70.2 HSP70.3 Hsp70Aa-PA Hsp70Bb-PA HSPA2 HSPA4 Hspa70 HST inducible heat shock protein 70 IRP94 ischemia responsive 94 kDa protein LP05203p LP05233p LP08776p protein 70 spermatid-specific heat shock protein 70 testis-specific heat shock protein-related gene hst70 |
| Molecular Weight(MW) | 94KD |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P34932 |
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WB
Western blot analysis using HSP70 antibody against HepG2(1),Cos7(2) cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ICC/IF
Immunofluorescence analysis of A549 cells using HSP70 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer 2 tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IF-P
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:300 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:300 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.
WB
Western blot analysis using HSP70 antibody against Mouse brain tissue(1),Mouse testes tissue(2),Mouse liver tissue(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500 IF-P:1:200-1:2000 |
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| Form | Liquid |
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| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
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