| 存储条件 |
|---|
| Product Name | Anti-TLE4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| IHC:1:200-1:2000 WB:1:1000-1:2000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TLE4 |
|---|---|
| Gene Synonyms | 2610103N05Rik 5730411M05Rik BCE-1 BCE1 C230057C06Rik E(spI) E(spl) ESG ESG1 ESG2 ESG3 ESG4 ESTM13 ESTM14 Gm32024 Grg-1 Grg-4 GRG1 GRG2 GRG3 Grg3a Grg3b GRG4 HsT18976 mKIAA1547 mKIAA4188 TLE-1 Tle4l |
| Gene Full Name | TLE family member 4, transcriptional corepressor |
| Gene Summary | Predicted to enable transcription corepressor activity. Predicted to be involved in negative regulation of canonical Wnt signaling pathway. Predicted to act upstream of or within Wnt signaling pathway; cellular response to leukemia inhibitory factor; and negative regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of beta-catenin-TCF complex. |
| Alternative Names | B lymphocyte gene 1 enhancer of split groucho 3 enhancer of split groucho 4 enhancer of split groucho-like protein 1 enhancer of split groucho-like protein 2 enhancer of split groucho-like protein 3 ESG-1 ESG-2 ESG-3 ESG-4 grg-3 groucho-related protein groucho-related protein 1 groucho-related protein 4 similar to Homo sapiens BCE-1 transcriptional corepressor transducin like enhancer of split 1 transducin like enhancer of split 2 transducin like enhancer of split 3 transducin like enhancer of split 4 transducin-like enhancer of split 1 (E(sp1) homolog Drosophila) transducin-like enhancer of split 1 homolog of Drosophila E(spl) transducin-like enhancer of split 2 (E(sp1) homolog Drosophila) transducin-like enhancer of split 2 homolog of Drosophila E(sp1) transducin-like enhancer of split 2 homolog of Drosophila E(spl) transducin-like enhancer of split 3 (E(sp1) homolog Drosophila) transducin-like enhancer of split 3 homolog of Drosophila E(sp1) transducin-like enhancer of split 3 homolog of Drosophila E(spl) transducin-like enhancer of split 4 (E(sp1) homolog Drosophila) transducin-like enhancer of split 4 (homolog of Drosophila E(spl)) - like transducin-like enhancer of split 4 homolog of Drosophila E(sp1) transducin-like enhancer of split 4 homolog of Drosophila E(spl) |
| Molecular Weight(MW) | 84 kDa(observed:60kDa) |
| Cellular Localization | Nucleus |
| SwissProt ID | Q04727 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using TLE4 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using TLE4 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
WB
Western blot analysis using TLE4 antibody against Mouse liver tissue(1),Rat liver tissue(2). 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | IHC:1:200-1:2000 WB:1:1000-1:2000 |
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| Form | Liquid |
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| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
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