| 存储条件 |
|---|
| Product Name | RPA1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSSB; RF-A; RP-A; REPA1; RPA70; MST075 |
| Immunogen | Purified recombinant fragment of human RPA1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | Unknown |
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMonkey |
| Purification | Unpurified |
| Alternative Names | HSSB RF-A RP-A REPA1 RPA70 MST075 |
|---|---|
| Molecular Weight(MW) | 68.1kDa |
| Entrez Gene | 6117 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using RPA1 mAb against human RPA1 (AA: 308-513) recombinant protein. (Expected MW is 48.3 kDa)
Application
Figure 2: Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using RPA1 mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded prostate tissues using RPA1 mouse mAb with DAB staining.
Application
Figure 5: Immunofluorescence analysis of HeLa cells using RPA1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 6: Flow cytometric analysis of Jurkat cells using RPA1 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
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