| 存储条件 |
|---|
| Product Name | CYP3A4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1 |
| Immunogen | Purified recombinant fragment of human CYP3A4 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | Unknown |
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Purification | Unpurified |
| Alternative Names | HLP CP33 CP34 CYP3A NF-25 CYP3A3 P450C3 CYPIIIA3 CYPIIIA4 P450PCN1 |
|---|---|
| Molecular Weight(MW) | 57.3kDa |
| Entrez Gene | 1576 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using CYP3A4 mAb against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)
Application
Figure 2: Western blot analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CYP3A4 mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded human brain tissues using CYP3A4 mouse mAb with DAB staining.
Application
Figure 5: Immunofluorescence analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 6: Flow cytometric analysis of HepG2 cells using CYP3A4 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
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