| 存储条件 |
|---|
| Product Name | CK5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT5; K5; DDD; EBS2; KRT5A |
| Immunogen | Purified recombinant fragment of human CK5 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseMonkey |
| Alternative Names | KRT5 K5 DDD EBS2 KRT5A |
|---|---|
| Molecular Weight(MW) | 62.3kDa |
| Entrez Gene | 3852 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using CK5 mAb against human CK5 (AA: 158-272) recombinant protein. (Expected MW is 33.3 kDa)
Application
Figure 2: Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CK5 mouse mAb with DAB staining.
Application
Figure 4: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CK5 mouse mAb with DAB staining.
Application
Figure 5: Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 6: Immunofluorescence analysis of HeLa cells using CK5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 7: Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
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