| 存储条件 |
|---|
| Product Name | PTPN1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTP1B |
| Immunogen | Purified recombinant fragment of human PTPN1 (AA: 40-246) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Alternative Names | PTP1B |
|---|---|
| Molecular Weight(MW) | 50kDa |
| Entrez Gene | 5770 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using PTPN1 mAb against human PTPN1 (AA: 40-246) recombinant protein. (Expected MW is 50 kDa)
Application
Figure 2: Western blot analysis using PTPN1 mAb against HEK293 (1) and PTPN1 (AA: 40-246)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using PTPN1 mouse mAb against Jurkat (1), MCF-7 (2) cell lysate.
Application
Figure 4:Immunofluorescence analysis of Hela cells using PTPN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5: Flow cytometric analysis of Jurkat cells using PTPN1 mouse mAb (green) and negative control (red).
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PTPN1 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
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