| 存储条件 |
|---|
| Product Name | PPP1CB |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PP1B; PP-1B; PPP1CD; PP1beta |
| Immunogen | Purified recombinant fragment of human PPP1CB (AA: 174-327) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Alternative Names | PP1B PP-1B PPP1CD PP1beta |
|---|---|
| Molecular Weight(MW) | 37.2kDa |
| Entrez Gene | 5500 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 1: Western blot analysis using PPP1CB mAb against human PPP1CB (AA: 174-327) recombinant protein. (Expected MW is 43.2 kDa)
Application
Figure 2: Western blot analysis using PPP1CB mAb against HEK293 (1) and PPP1CB (AA: 174-327)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 3: Western blot analysis using PPP1CB mouse mAb against Jurkat (1), A431 (2), Hela (3), HepG2 (4), HEK293 (5), MCF-7 (6) cell lysate.
Application
Figure 4: Immunofluorescence analysis of MCF-7 cells using PPP1CB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 5: Flow cytometric analysis of Jurkat cells using PPP1CB mouse mAb (green) and negative control (red).
Application
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PPP1CB mouse mAb with DAB staining.
Application
Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PPP1CB mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
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