| 存储条件 |
|---|
| Product Name | PDPK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDK1; PDPK2; PDPK2P; PRO0461 |
| Immunogen | Purified recombinant fragment of human PDPK1 (AA: 457-556 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Alternative Names | PDK1 PDPK2 PDPK2P PRO0461 |
|---|---|
| Molecular Weight(MW) | 63.2kDa |
| Entrez Gene | 5170 |
|---|
Application
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application
Figure 2:Western blot analysis using PDPK1 mAb against human PDPK1 (AA: 457-556) recombinant protein. (Expected MW is 37.9 kDa)
Application
Figure 3:Western blot analysis using PDPK1 mAb against HEK293 (1) and PDPK1 (AA: 457-556)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), and U937 (3) cell lysate.
Application
Figure 5:Flow cytometric analysis of Hela cells using PDPK1 mouse mAb (green) and negative control (red).
Application
Figure 6:Immunofluorescence analysis of Hela cells using PDPK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
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