| 存储条件 |
|---|
| Product Name | Mouse Monoclonal Antibody to PRKAA2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human PRKAA2 (AA: 453-552) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCMICC/IFIHCWB |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Summary | The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008] |
|---|---|
| Alternative Names | AMPK AMPK2 PRKAA AMPKa2 |
| Molecular Weight(MW) | 62.3kDa |
| Entrez Gene | 5563 |
|---|
Elisa
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
WB
Western blot analysis using PRKAA2 mAb against human *** (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)
WB
Western blot analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.
WB
Western blot analysis using PRKAA2 mouse mAb against HEK293 (1) and COS7 (2) cell lysate.
FC
Flow cytometric analysis of Jurkat cells using PRKAA2 mouse mAb (green) and negative control (red).
IHC
Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PRKAA2 mouse mAb with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Mouse kidney using PRKAA2 mouse mAb with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Rat kidney using PRKAA2 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
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