| 存储条件 |
|---|
| Product Name | NAGR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M |
| Immunogen | Purified recombinant fragment of human NAGR1 (AA: 17-161) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | Unknown |
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Purification | Unpurified |
| Alternative Names | CEAR HNRPM HTGR1 NAGR1 HNRPM4 HNRNPM4 hnRNP M |
|---|---|
| Molecular Weight(MW) | 77.5kDa |
| Entrez Gene | 4670 |
|---|
Application
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application
Figure 2:Western blot analysis using NAGR1 mAb against human NAGR1 (AA: 17-161) recombinant protein. (Expected MW is 42.2 kDa)
Application
Figure 3:Western blot analysis using NAGR1 mAb against HEK293 (1) and NAGR1 (AA: 17-161)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Figure 4:Western blot analysis using NAGR1 mouse mAb against Raji (1), Hela (2), NIH/3T3 (3), A431 (4), A549 (5), HepG2 (6), PC-12 (7), and U251 (8) cell lysate.
Application
Figure 5:Immunofluorescence analysis of Hela cells using NAGR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 6:Immunofluorescence analysis of Hela cells using NAGR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Application
Figure 7:Flow cytometric analysis of HL-60 cells using NAGR1 mouse mAb (green) and negative control (red).
Application
Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NAGR1 mouse mAb with DAB staining.
Application
Figure 9:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NAGR1 mouse mAb with DAB staining.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Purified antibody in PBS with 0.05% sodium azide |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
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