| 存储条件 |
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| Product Name | MDM2 [3G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTFS antibody Double minute 2 protein antibody E3 ubiquitin-protein ligase Mdm2 antibody Hdm 2 antibody Hdm2 antibody HDMX antibody MDM 2 antibody MDM2 antibody MDM2 oncogene E3 ubiquitin protein ligase antibody Mdm2 p53 E3 ubiquitin protein ligase homolog antibody Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody MDM2_HUMAN antibody MDM2BP antibody Mouse Double Minute 2 antibody MTBP antibody Murine Double Minute Chromosome 2 antibody Oncoprotein Mdm2 antibody p53 Binding Protein Mdm2 antibody p53-binding protein Mdm2 antibody Ubiquitin protein ligase E3 Mdm2 antibody Ubiquitin protein ligase E3 Mdm2 antibody |
| Product description | p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis. |
| Immunogen | Amino acids 154-167 of MDM2 of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | ACTFS antibody Double minute 2 protein antibody E3 ubiquitin-protein ligase Mdm2 antibody Hdm 2 antibody Hdm2 antibody HDMX antibody MDM 2 antibody MDM2 antibody MDM2 oncogene E3 ubiquitin protein ligase antibody Mdm2 p53 E3 ubiquitin protein ligase homolog antibody Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody MDM2_HUMAN antibody MDM2BP antibody Mouse Double Minute 2 antibody MTBP antibody Murine Double Minute Chromosome 2 antibody Oncoprotein Mdm2 antibody p53 Binding Protein Mdm2 antibody p53-binding protein Mdm2 antibody Ubiquitin protein ligase E3 Mdm2 antibody Ubiquitin protein ligase E3 Mdm2 antibody |
|---|---|
| Molecular Weight(MW) | 55/90 kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | Q00987 |
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Application
Fig1: Western blot analysis of MDM2 expression in U-2OS (A), A-673 (B) and RAW 264.7 (C) whole cell lysates.
Application
Fig2： Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue (A). Immunofluorescence staining of methanol-fixed rat embryo fibroblasts showing nuclear localization (B).| Positive Control | U-2OS, A-673, RAW264.7, human breast carcinoma tissue. |
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| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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