| 存储条件 |
|---|
| Product Name | Gli2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CJS antibody Gli 2 antibody GLI family zinc finger 2 antibody GLI Kruppel family member GLI2 antibody GLI2 antibody GLI2_HUMAN antibody Glioma associated oncogene family zinc finger antibody HPE9 antibody Oncogene GLI2 antibody PHS2 antibody Tax helper protein 1 antibody Tax helper protein 2 antibody Tax helper protein antibody Tax responsive element 2 holding protein antibody Tax responsive element 25 bp sequence binding protein antibody THP antibody THP1 antibody THP2 antibody Zinc finger protein GLI2 antibody |
| Product description | It has long been known that the overexpression of either Wnt-1 or the GLI proteins results in cancer; however, the molecular basis for this transformation was poorly understood. The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins. The Drosophila segment polarity gene hedgehog (hh) encodes a secreted protein that appears to function in embryonic and imaginal disc patterning. The ptc gene, also identified as a Drosophila segment polarity gene, encodes the transmembrane protein patched, the expression of which is precisely regulated during embryonic development. Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, while patched functions as a repressor opposing the effects of hedgehog. Mutations in the ptc gene, which result in unregulated hedgehog signaling, have been correlated with the most common type of cancer, basal cell carcinoma, which affects 750,000 individuals annually in the United States alone. |
| Immunogen | Amino acids 841-1140 mapping near the C-terminus of GLI-2 of human origin |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CJS antibody Gli 2 antibody GLI family zinc finger 2 antibody GLI Kruppel family member GLI2 antibody GLI2 antibody GLI2_HUMAN antibody Glioma associated oncogene family zinc finger antibody HPE9 antibody Oncogene GLI2 antibody PHS2 antibody Tax helper protein 1 antibody Tax helper protein 2 antibody Tax helper protein antibody Tax responsive element 2 holding protein antibody Tax responsive element 25 bp sequence binding protein antibody THP antibody THP1 antibody THP2 antibody Zinc finger protein GLI2 antibody |
|---|---|
| Molecular Weight(MW) | Isoforms 5/α/β/γ/δ:168/133/132/88/86 kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P10070 |
|---|
Application
Fig1: Western blot analysis of GLI-2 expression in K-562 (A), TF-1 (B), PC-3 (C), Jurkat (E) and MDA-MB-231 (F) nuclear extracts and F9 whole cell lysate (D).| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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