| 存储条件 |
|---|
| Product Name | Gli1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gli 1 antibody GLI antibody GLI family zinc finger 1 antibody GLI Kruppel family member 1 antibody gli1 antibody GLI1_HUMAN antibody Glioma associated oncogene 1 antibody Glioma associated oncogene homolog 1 (zinc finger protein) antibody Glioma associated oncogene homolog antibody Glioma-associated oncogene antibody Oncogene GLI antibody Zfp 5 antibody Zfp5 antibody Zinc finger protein GLI 1 antibody Zinc finger protein GLI1 antibody |
| Product description | It has long been known that the overexpression of either Wnt-1 or the GLI proteins results in cancer; however, the molecular basis for this transformation was poorly understood. The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins. The Drosophila segment polarity gene hedgehog (hh) encodes a secreted protein that appears to function in embryonic and imaginal disc patterning. The ptc gene, also identified as a Drosophila segment polarity gene, encodes the transmembrane protein patched, the expression of which is precisely regulated during embryonic development. Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, while patched functions as a repressor opposing the effects of hedgehog. Mutations in the ptc gene, which result in unregulated hedgehog signaling, have been correlated with the most common type of cancer, basal cell carcinoma, which affects 750,000 individuals annually in the United States alone. |
| Immunogen | Amino acids 781-1080 of GLI-1 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Gli 1 antibody GLI antibody GLI family zinc finger 1 antibody GLI Kruppel family member 1 antibody gli1 antibody GLI1_HUMAN antibody Glioma associated oncogene 1 antibody Glioma associated oncogene homolog 1 (zinc finger protein) antibody Glioma associated oncogene homolog antibody Glioma-associated oncogene antibody Oncogene GLI antibody Zfp 5 antibody Zfp5 antibody Zinc finger protein GLI 1 antibody Zinc finger protein GLI1 antibody |
|---|---|
| Molecular Weight(MW) | Predicted：118 kDa ,observed：114-173 kDa. |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P08151 |
|---|
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of ductus seminiferus cells and cytoplasmic staining of Leydig cells at low (A) and high (B) magnification.Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of ductus seminiferus cells and cytoplasmic staining of Leydig cells at low (A) and high (B) magnification.| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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