| 存储条件 |
|---|
| Product Name | c-Jun |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activator protein 1 antibody AP 1 antibody AP1 antibody cJun antibody Enhancer Binding Protein AP1 antibody Jun Activation Domain Binding Protein antibody JUN antibody Jun oncogene antibody JUN protein antibody Jun proto oncogene antibody JUN_HUMAN antibody JUNC antibody Oncogene JUN antibody p39 antibody Proto oncogene c jun antibody Proto oncogene cJun antibody Proto-oncogene c-jun antibody Transcription Factor AP 1 antibody Transcription factor AP-1 antibody Transcription Factor AP1 antibody V jun avian sarcoma virus 17 oncogene homolog antibody V jun sarcoma virus 17 oncogene homolog (avian) antibody V jun sarcoma virus 17 oncogene homolog antibody V-jun avian sarcoma virus 17 oncogene homolog antibody vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody hide |
| Product description | Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA). |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Activator protein 1 antibody AP 1 antibody AP1 antibody cJun antibody Enhancer Binding Protein AP1 antibody Jun Activation Domain Binding Protein antibody JUN antibody Jun oncogene antibody JUN protein antibody Jun proto oncogene antibody JUN_HUMAN antibody JUNC antibody Oncogene JUN antibody p39 antibody Proto oncogene c jun antibody Proto oncogene cJun antibody Proto-oncogene c-jun antibody Transcription Factor AP 1 antibody Transcription factor AP-1 antibody Transcription Factor AP1 antibody V jun avian sarcoma virus 17 oncogene homolog antibody V jun sarcoma virus 17 oncogene homolog (avian) antibody V jun sarcoma virus 17 oncogene homolog antibody V-jun avian sarcoma virus 17 oncogene homolog antibody vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody hide |
|---|---|
| Molecular Weight(MW) | 39kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P05412 |
|---|
Application
Fig1: Western blot analysis of c-Jun phosphorylation in nontransfected (A,D), untreated mouse c-Jun transfected (B,E) and lambda protein phosphatase treated human c-Jun transfected(C,F) 293T whole cell lysates.
Application
Fig2： Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. (A). Immunoperoxidase staining of formalin-fixed, paraffinembedded normal human prostate tissue showing nuclear localization (B).| Positive Control | Hela, 293T, human prostate tissue |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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