| 存储条件 |
|---|
| Product Name | MLH1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COCA 2 antibody COCA2 antibody DNA mismatch repair protein Mlh1 antibody FCC 2 antibody FCC2 antibody hMLH 1 antibody hMLH1 antibody HNPCC 2 antibody HNPCC antibody HNPCC2 antibody MGC5172 antibody MLH 1 antibody MLH1 antibody MLH1_HUMAN antibody MutL homolog 1 (E. coli) antibody MutL homolog 1 antibody MutL homolog 1 colon cancer nonpolyposis type 2 antibody MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antibody MutL protein homolog 1 antibody MutL, E. coli, homolog of, 1 antibody |
| Product description | MLH1 is a DNA mismatch repair protein that heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, and then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in the presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the mismatch strand. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may recruit DNA polymerase III to the site of the MMR. Defects in MLH1 are the cause of hereditary nonpolyposis colorectal cancer type 2 (HNPCC2). Most patients with HNPCC have mutations in either the MLH1 or MSH2 genes (1). |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFCIP |
| WB:1:1,000 IP:1:10-100 FC:1:10-100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | COCA 2 antibody COCA2 antibody DNA mismatch repair protein Mlh1 antibody FCC 2 antibody FCC2 antibody hMLH 1 antibody hMLH1 antibody HNPCC 2 antibody HNPCC antibody HNPCC2 antibody MGC5172 antibody MLH 1 antibody MLH1 antibody MLH1_HUMAN antibody MutL homolog 1 (E. coli) antibody MutL homolog 1 antibody MutL homolog 1 colon cancer nonpolyposis type 2 antibody MutL homolog 1 colon cancer nonpolyposis type 2 (E. coli) antibody MutL protein homolog 1 antibody MutL E. coli homolog of 1 antibody |
|---|---|
| Molecular Weight(MW) | 85 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P40692 |
|---|
Application
Fig1: Western blot analysis on (A) 293, (B) HeLa, (C) A431, and (D) SW480 cell lysates using anti-MLH1.| Positive Control | Hela, 293, A431, SW480. |
|---|---|
| Application Notes | WB:1:1,000 IP:1:10-100 FC:1:10-100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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