| 存储条件 |
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| Product Name | Cytochrome C |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYC antibody CYC_HUMAN antibody CYCS antibody Cytochrome c antibody Cytochrome c somatic antibody HCS antibody THC4 antibody |
| Product description | Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CYC antibody CYC_HUMAN antibody CYCS antibody Cytochrome c antibody Cytochrome c somatic antibody HCS antibody THC4 antibody |
|---|---|
| Molecular Weight(MW) | 12kDa |
| Cellular Localization | Mitochondrion intermembrane space |
| SwissProt ID | P99999 |
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Application
Fig1: Western blot analysis on mouse brain(1) mouse heart(2) and mouse kidney(3) cell lysates using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig2： Immunocytochemical staining of Hela cells using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig3： Immunocytochemical staining of HepG2 cells using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig4： Immunocytochemical staining of MCF-7 cells using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig5： Immunocytochemical staining of 293 cells using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig6： Immunohistochemical analysis of paraffin- embedded human spleen tissue using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig7： Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig8： Immunohistochemical analysis of paraffin- embedded mouse liver tissue using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig9： Immunohistochemical analysis of paraffin- embedded mouse spleen tissue using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig10： Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig11： Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig12： Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-Cytochrome C rabbit polyclonal antibody.
Application
Fig13： Flow cytometric analysis of Hela cells with Cytochrome C antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, HepG2, 293, MCF-7, human spleen tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse spleen tissue, mouse heart tissue, mouse kidney tissue. |
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| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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