| 存储条件 |
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| Product Name | Caspase-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP-1 antibody CASP1 antibody CASP1_HUMAN antibody Caspase 1 antibody Caspase-1 subunit p10 antibody ICE antibody IL-1 beta-converting enzyme antibody IL-1BC antibody IL1 beta converting enzyme antibody IL1B convertase antibody Interleukin 1 beta convertase antibody Interleukin 1B converting enzyme antibody Interleukin-1 beta convertase antibody Interleukin-1 beta-converting enzyme antibody p45 antibody |
| Product description | Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Caspase-1 promotes maturation of interleukin IL-1β and interleukin18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflamatory cytokines. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. The highly conserved family of caspases mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. The human Caspase-1 gene maps to chromosome 2q14 and encodes a cytoplasmic protein expressed in liver, heart, skeletal muscle kidney and testis. Caspase-1 has been implicated in inflammation, septic shock, and other situations such as wound healing and the growth of certain leukemias. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICCIHCFC |
| ICC:1:200~1:500 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CASP-1 antibody CASP1 antibody CASP1_HUMAN antibody Caspase 1 antibody Caspase-1 subunit p10 antibody ICE antibody IL-1 beta-converting enzyme antibody IL-1BC antibody IL1 beta converting enzyme antibody IL1B convertase antibody Interleukin 1 beta convertase antibody Interleukin 1B converting enzyme antibody Interleukin-1 beta convertase antibody Interleukin-1 beta-converting enzyme antibody p45 antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P29466 |
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Application
Fig1: ICC staining Caspase-1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- Caspase-1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Caspase-1 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Jurkat cells with Caspase-1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | HepG2, human lung tissue, human liver tissue, Jurkat. |
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| Application Notes | ICC:1:200~1:500 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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