| 存储条件 |
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| Product Name | TCF4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bHLHb19 antibody Class B basic helix-loop-helix protein 19 antibody E2 2 antibody FECD3 antibody Immunoglobulin transcription factor 2 antibody ITF 2 antibody ITF-2 antibody ITF2 antibody ITF2_HUMAN antibody MGC149723 antibody MGC149724 antibody PTHS antibody SEF 2 antibody SEF-2 antibody SEF2 1 antibody SEF2 1A antibody SEF2 1B antibody SEF2 1D antibody SEF2 antibody SL3 3 enhancer factor 2 antibody SL3-3 enhancer factor 2 antibody TCF 4 antibody TCF-4 antibody TCF4 antibody Transcription factor 4 antibody Transcription factor 4, isoform C antibody Transcription factor 4, isoform D antibody Transcription factor 4, isoform E antibody Transcription factor 4, isoform L antibody Transcription factor 4, isoform M antibody Transcription factor 4, isoform R antibody |
| Product description | TCF7L2 is a transcription factor influencing the transcription of several genes thereby exerting a large variety of functions within the cell. It is a member of the Wnt signaling pathway. Stimulation of the pathway leads to the association of β-catenin with BCL9, translocation to the nucleus, and association with TCF7L2, which in turn results in the activation of Wnt target genes, specifically repressing proglucagon synthesis in enteroendocrine cells. TCF7L2 is implicated in a large variety of diseases. Several single nucleotide polymorphisms are associated with type 2 diabetes. In European populations it was found to be a major determinant of type 2 risk. A frameshift mutation of TCF7L2 is implicated in colorectal cancer .Variants of the gene are most likely involved in many other cancer types |
| Immunogen | Synthetic peptide (KLH-coupled) within human TCF4 N-terminal. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCICCFC |
| WB:1:1,000-1:2,000 ICC:1:500-1:2,000 IHC:1:100-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | bHLHb19 antibody Class B basic helix-loop-helix protein 19 antibody E2 2 antibody FECD3 antibody Immunoglobulin transcription factor 2 antibody ITF 2 antibody ITF-2 antibody ITF2 antibody ITF2_HUMAN antibody MGC149723 antibody MGC149724 antibody PTHS antibody SEF 2 antibody SEF-2 antibody SEF2 1 antibody SEF2 1A antibody SEF2 1B antibody SEF2 1D antibody SEF2 antibody SL3 3 enhancer factor 2 antibody SL3-3 enhancer factor 2 antibody TCF 4 antibody TCF-4 antibody TCF4 antibody Transcription factor 4 antibody Transcription factor 4 isoform C antibody Transcription factor 4 isoform D antibody Transcription factor 4 isoform E antibody Transcription factor 4 isoform L antibody Transcription factor 4 isoform M antibody Transcription factor 4 isoform R antibody |
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| Molecular Weight(MW) | 40-80 kDa (different isoforms) |
| Cellular Localization | Nucleus |
Application
Fig1: Western blot analysis of TCF4 on different cell lysates using anti-TCF4 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining TCF4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TCF4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-TCF4 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TCF4 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TCF4 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-TCF4 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of HepG2 cells with TCF4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Hela, HepG2, HCT116, SW480, rat kidney tissue, human tonsil tissue, human colon cancer tissue, human breast cancer tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:500-1:2,000 IHC:1:100-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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