| 存储条件 |
|---|
| Product Name | LRRK1 [5B1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIAA1790 antibody Leucine rich repeat serine/threonine protein kinase 1 antibody Leucine-rich repeat serine/threonine-protein kinase 1 antibody LRRK 1 antibody Lrrk1 antibody LRRK1_HUMAN antibody RIPK6 antibody Roco1 antibody |
| Product description | Leucine-rich repeat serine/threonine-protein kinase 1 is an enzyme that in humans is encoded by the LRRK1 gene. Formation of LRRK1:EGF-R complexes result in LRRK cellular translocation: LRRK1 traffics together with EGF-R to endosomes upon stimulation of the receptor with EGF. LRRK1 is related to the familial Parkinsonism gene product Park8/LRRK2, and contains a Ras of complex proteins (ROC) GTPase domain and a MAPKKK-like kinase domain. LRRK1 participates in the intercellular trafficking of the epidermal growth factor (EGF) receptor. LRRK1 is activated by the sequential phosphorylation of 2 mitotic kinases, PLK1 and CDK1. Importantly, this PLK1-dependent activation of LRRK1 is required for the regulation of mitotic spindle orientation.LRRK1 regulates spindle orientation in a manner dependent on its kinase activity. Furthermore, the spindle misorientation caused by PLK1 inactivation is rescued by expression of the phospho-mimicking mutant LRRK1(S1790D). LRRK1 regulates spindle orientation downstream of PLK1. |
| Immunogen | Peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 IHC:1:50-1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | KIAA1790 antibody Leucine rich repeat serine/threonine protein kinase 1 antibody Leucine-rich repeat serine/threonine-protein kinase 1 antibody LRRK 1 antibody Lrrk1 antibody LRRK1_HUMAN antibody RIPK6 antibody Roco1 antibody |
|---|---|
| Molecular Weight(MW) | 225kDa, additional band 60kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of LRRK1 on A549 lysate using anti-LRRK1 antibody at 1/100 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.| Positive Control | A549, mouse brain tissue, mouse kidney tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500 IHC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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