Anti-GADD34抗体
参阅全部 GADD34 一抗
山羊多克隆抗体to GADD34
Goat
适用于: IHC-P, WBmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Human GADD34 aa 650 to the C-terminus (C terminal) (Cysteine residue). NP_055145.3.
Database link: O75807-1
WB: HepG2 cell lysate. IHC-P: Human liver tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab9869于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | (1) | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). 1 hour primary incubation is recommended for this product. |
Entrez Gene: 23645 Human
Entrez Gene: 17872 Mouse
Omim: 611048 Human
SwissProt: O75807 Human
SwissProt: P17564 Mouse
Unigene: 631593 Human
Unigene: 4048 Mouse
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Western blot - Anti-GADD34 antibody (ab9869)
Anti-GADD34 antibody (ab9869) at 0.1 µg/ml + HEPG2 cell lysate at 35 µg
Predicted band size: 75 kDa
Primary incubation for 1 hour. Detection by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (ab9869)
IHC image of ab9869 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9869, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (ab9869)
ab9869 staining GADD34 in human liver sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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