Anti-MCP1抗体[2.2-4H5-1A11]
参阅全部 MCP1 一抗
小鼠单克隆抗体[2.2-4H5-1A11] to MCP1
Mouse
适用于: WB, IHC-P, ELISA, Sandwich ELISAmore details
与反应: Human, Recombinant fragment
Recombinant full length protein corresponding to Human MCP1. Recombinant hMCAF/MCP-1 (human Macrophage/Monocyte chemotactic protein-1)
Database link: P13500
WB: Recombinant human MCP1 protein (ab9670).
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab73680
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Protein A purified
单克隆
2.2-4H5-1A11
unknown
IgG1
kappa
Abpromise™承诺保证使用ab9858于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. | |
ELISA | Use at an assay dependent concentration. | |
Sandwich ELISA | Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MCP1 (ab9669). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9669 as Detection. |
Entrez Gene: 6347 Human
Omim: 158105 Human
SwissProt: P13500 Human
Unigene: 303649 Human
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Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (ab9858)
In a sandwich ELISA (assuming 100 μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-1 when used with Biotin Anti-MCP1 antibody (ab271250) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (ab9858)
Standard curve for MCP1 (Analyte: ab73866); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5µg/ml.
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