Anti-TGF beta 1抗体
参阅全部 TGF beta 1 一抗
兔多克隆抗体to TGF beta 1
Rabbit
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
适用于: WB, ELISAmore details
与反应: Human
预测可用于: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, Pig不与反应: Dog
Synthetic peptide corresponding to Human TGF beta 1.
Sequence:
FSSTEKN-C
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1.13 mg/ml
100 µg 浓度为 0.43 mg/ml
Immunogen affinity purified
Rabbit Anti-TGF beta 1 affinity purified antibody is directed against human TGFb1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
多克隆
IgG
Abpromise™承诺保证使用ab9758于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) | 1/100 - 1/500. In reduced samples, the expected bands are 12 kDa (mature protein) and 44 kDa (unprocessed protein). Expected band at 25 kDa in non-reduced samples (homodimer of mature protein). Glycosylations may also cause unprocessed protein to run a few kDa higher (around 50 kDa). TGFB1 also heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. Read More |
ELISA | 1/10000 - 1/20000. |
Entrez Gene: 7040 Human
Omim: 190180 Human
SwissProt: P01137 Human
Unigene: 645227 Human
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Western blot - Anti-TGF beta 1 antibody (ab9758)
All lanes : Anti-TGF beta 1 antibody (ab9758) at 1/200 dilution
Lane 1 : Reduced human recombinant TGF beta 1
Lane 2 : Non-reduced human recombinant TGF beta 1
Lysates/proteins at 0.25 µg per lane.
Secondary
All lanes : Peroxidase-conjugated anti-rabbit IgG at 1/40000 dilution
Observed band size: 12,25 kDawhy is the actual band size different from the predicted?
Incubated with the primary antibody overnight at 4°C.
Incubated with the secondary antibody for 30 min at room temperature.
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