Anti-PDGF BB抗体
参阅全部 PDGF B 一抗
兔多克隆抗体to PDGF BB
Rabbit
适用于: WBmore details
与反应: Human
Recombinant full length protein corresponding to Human PDGF B. Highly pure (>98%) recombinant hPDGF-BB (human Platelet Derived Growth Factor)
Database link: P01127
WB: Recombinant human PDGF B protein (ab9706).
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab107101
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
No preservative, sterile filtered
Immunogen affinity purified
多克隆
unknown
unknown
Abpromise™承诺保证使用ab9704于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) | Use at an assay dependent concentration. To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More |
Entrez Gene: 5155 Human
Omim: 190040 Human
SwissProt: P01127 Human
Unigene: 1976 Human
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Western blot - Anti-PDGF BB antibody (ab9704)
Anti-PDGF BB antibody (ab9704) at 0.2 µg/ml + A-431 (Human) Whole Cell Lysate (ab52255) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 18,29 kDawhy is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible dimer)
The 29kDa form is the propeptide.
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