Recombinant human EGF protein (Animal Free)

• 产品名称

  重组人EGF蛋白(Animal Free)
  参阅全部 EGF 蛋白酶

• 生物活性

  The ED₅₀,as determined by a cell proliferation assay using balb/c 3T3 cells, is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

• 纯度

  >= 98 % SDS-PAGE.
  Sterile filtered. Greater than 98% pure by HPLC analyses.
  

• 内毒素水平

  < 0.010 Eu/µg

• 表达系统

  Escherichia coli

• Accession

  Q6QBS2

• 蛋白长度

  Full length protein

• 无动物成分

  Yes

• 性质

  Recombinant

• 
  

• 种属

  Human

• 序列

  NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

• 预测分子量

  6 kDa

• 氨基酸

  971 to 1023

• 额外的序列信息

  ab9697 is a globular protein containing 53 amino acid residues, including 2 intramolecular disulfide bonds.

Our Abpromise guarantee covers the use of ab9697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  Western blot

  Cellular Activation

  SDS-PAGE

  HPLC

• 形式

  Lyophilized

• 补充说明

  Manufactured using all non-animal reagents.

• 稳定性和存储

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  This product is an active protein and may elicit a biological response in vivo, handle with caution.

• 复溶

  Centrifuge the vial prior to opening. Reconstitute in 500ul sterile filtered water to a concentration of 1mg/ml.

别名

• Beta urogastrone

• beta-urogastrone

• EGF

• EGF_HUMAN

• Epidermal growth factor

• HOMG4

• OTTHUMP00000219721

• OTTHUMP00000219722

• Pro epidermal growth factor

• URG

• Urogastrone

• 

  Western blot - Recombinant human EGF protein (Animal Free) (ab9697)This image is courtesy of an anonymous Abreview

  All lanes :
  
  Lane 1 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium
  Lane 2 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 25 ng/ml active EGF
  Lane 3 : Whole cell lysate of human skin fibroblasts starved overnight in serum-free medium and then incubated for 30 min with 50 ng/ml active EGF
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : An HRP-conjugatede Goat anti-rabbit IgG polyclonal at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Performed under non-reducing conditions.
  
  Observed band size: 42,44 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 1 second
  
  
  Blocking Step: 5% milk for 1 hour at 25°C
  
  

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Recombinant human EGF protein (Animal Free) (ab9697)

  Immunocytochemistry/ Immunofluorescence  analysis of A431 (Human epidermoid carcinoma cell line) cells labeling EGFR with ab9697 at 1/100, 3 μg/ml. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, a AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000, 2 μg/ml. Cells were counterstained with ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200, 2.5 μg/ml. Nuclear stain was DAPI (blue).

  The green staining on the membrane was increased in the EGF (100ng/ml, 10min) treated A431 cells when compared with A431 cells without treatment. After LP treatment, the green signaling was obviously decreased.

  For the pan antibody, there was no great difference after EGF (100ng/ml, 10min) or EGF (100ng/ml, 10min) + LP treatment. The data showed mostly membranous staining.