Anti-EGF抗体
参阅全部 EGF 一抗
兔多克隆抗体to EGF
Rabbit
适用于: WB, IHC-P, ELISA, Neutralising, Sandwich ELISAmore details
与反应: Human, Recombinant fragment
Recombinant full length protein corresponding to Human EGF. Highly pure (>98%) recombinant hEGF (human Epidermal Growth Factor)
Database link: P01133
Although some customers have been successful in IHC we no longer batch test in this application. For IHC validate antibody please see ab184265.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
No preservative, sterile filtered
Immunogen affinity purified
多克隆
IgG
unknown
Abpromise™承诺保证使用ab9695于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | Use a concentration of 0.1 - 0.2 µg/ml. To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western blot bands should be around 6.2 kDa. Read More |
| IHC-P | (1) | Use at an assay dependent concentration. |
| ELISA | Use at an assay dependent concentration. To detect hEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hEGF. Read More | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/ml), a concentration of 0.25 - 0.35 µg/ml of this antibody is required. | |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab18635 as Capture. |
Entrez Gene: 1950 Human
Omim: 131530 Human
SwissProt: P01133 Human
Unigene: 419815 Human
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Beta urogastrone antibody
beta-urogastrone antibody
EGF antibody
Western blot - Anti-EGF antibody (ab9695)
Primary ab9695 was used at a dilution of 0.2 ug/ml.
Lane 1: 250 ng Recombinant human EGF protein
Lane 2: 125 ng Recombinant human EGF protein
Sandwich ELISA - Anti-EGF antibody (ab9695)
Standard Curve for EGF (Analyte: ab51082) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [S-177] to EGF (ab18635) at 5ug/ml and Detector Antibody Rabbit polyclonal to EGF (ab9695) at 0.5ug/ml
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