Anti-G-CSF抗体
参阅全部 G-CSF 一抗
兔多克隆抗体to G-CSF
Rabbit
适用于: WB, Sandwich ELISAmore details
与反应: Human, Recombinant fragment
Recombinant fragment corresponding to Human G-CSF aa 1 to the C-terminus. Produced in E.coli. Isoform Short of G-CSF.
Database link: P09919-2
Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab216607.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
No preservative, sterile filtered
Immunogen affinity purified
多克隆
IgG
unknown
Abpromise™承诺保证使用ab9691于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) | Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More |
Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. This antigen affinity purified antibody, in conjunction with ab271256 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF |
Entrez Gene: 1440 Human
Omim: 138970 Human
SwissProt: P09919 Human
Unigene: 2233 Human
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Western blot - Anti-G-CSF antibody (ab9691)
Anti-G-CSF antibody (ab9691) at 1 µg/ml + Recombinant human G-CSF protein (ab73371) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 minutes
Sandwich ELISA - Anti-G-CSF antibody (ab9691)
Standard Curve for G-CSF (Analyte: ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml
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