Anti-Flt3 ligand/Flt3L抗体
参阅全部 Flt3 ligand/Flt3L 一抗
兔多克隆抗体to Flt3 ligand/Flt3L
Rabbit
适用于: Flow Cyt, WB, Neutralising, Sandwich ELISAmore details
与反应: Human, Recombinant fragment
Recombinant fragment corresponding to Human Flt3 ligand/Flt3L aa 1-200. Highly pure>98%. Produced in E. coli.
Database link: P49771
hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells
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Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
No preservative, sterile filtered
Immunogen affinity purified
hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells
多克隆
IgG
Abpromise™承诺保证使用ab9688于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. PubMed: 22897934 | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Neutralising | Use at an assay dependent concentration. Yields one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/ml) | |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. In conjunction with ab84261 rabbit polyclonal to Flt3 ligand/Flt3L (Biotin) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand/Flt3L. |
Entrez Gene: 2323 Human
Omim: 600007 Human
SwissProt: P49771 Human
Unigene: 428 Human
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Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (ab9688)
To detect human Flt3 ligand/Flt3L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84261 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Human Flt3-ligand (ab9688) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Flt3 ligand/Flt3L.
