Recombinant human Interferon gamma protein (Active)

• 产品名称

  重组人Interferon gamma蛋白(Active)
  参阅全部 Interferon gamma 蛋白酶

• 生物活性

  The ED₅₀ determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml, corresponding to a
  specific activity of ≥ 2 x 107 units/mg.

   

• 纯度

  > 98 % SDS-PAGE.
  >98%% HPLC analyses. Sterile filtered.
  

• 内毒素水平

  < 1.000 Eu/µg

• 表达系统

  Escherichia coli

• Accession

  Q14609

• 蛋白长度

  Full length protein

• 无动物成分

  No

• 性质

  Recombinant

• 
  

• 种属

  Human

• 序列

  MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

• 预测分子量

  17 kDa

• 氨基酸

  1 to 144

Our Abpromise guarantee covers the use of ab9659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  Western blot

  Functional Studies

  SDS-PAGE

  HPLC

• 形式

  Lyophilized

• 稳定性和存储

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  Constituent: PBS

  This product is an active protein and may elicit a biological response in vivo, handle with caution.

• 复溶

  Centrifuge vial prior to opening. Reconstitute in 100 µl 1x PBS, pH 8.0 to a concentration of 1.0 mg/ml. Do not vortex. Long term storage: Follow reconstitution with further dilution in a buffer containing a carrier protein (example; 0.1% BSA).

别名

• IF 1

• IFG

• IFI

• ifn g

• IFN gamma

• IFN immune

• IFN, immune

• IFN-gamma

• IFNG

• IFNG_HUMAN

• Immune interferon

• Interferon gamma

• Type II Interferon

• 

  Western blot - Recombinant human Interferon gamma protein (ab9659)

  All lanes : Anti-CXCL11 antibody [EPR21755-173] (ab216157) at 1/1000 dilution
  
  Lane 1 : THP-1 (hman monocytic leukemia cell line) whole cell lysate
  Lane 2 : THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 20 hours, whole cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 19 kDa
  Observed band size: 11 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 37 seconds
  
  
  

  Blocking/Dilution: 5% NFDM/TBST

  The expression profile observed is consistent with what has been described in the literature (PMID: 17142784).

• 

  Immunoprecipitation - Recombinant human Interferon gamma protein (ab9659)

  CXCL11 was immunoprecipitated from 0.35mg of THP-1 (human monocytic leukemia cell line) whole cell lysate with ab216157 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216157 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

  Lane 1: THP-1 (human monocytic leukemia cell line) treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate 10ug (Input).
  Lane 2: ab216157 IP in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate. 
  Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216157 in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.

  Blocking/Dilution buffer: 5% NFDM/TBST

• 

  Western blot - Recombinant human Interferon gamma protein (ab9659)

  All lanes : Anti-IP10 antibody [EPR20764] (ab214668) at 1/1000 dilution
  
  Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) culture supernatant
  Lane 2 : THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharides (LPS) for 24 hours, culture supernatant
  
  Lysates/proteins at 15 µl per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 19 kDa
  Observed band size: 12 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 15 seconds
  
  
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

  IP10 protein secretion can be induced by IFN-gamma treatment (PMID: 11907072).