Anti-Interferon gamma抗体
参阅全部 Interferon gamma 一抗
兔多克隆抗体to Interferon gamma
Rabbit
适用于: Sandwich ELISA, WBmore details
与反应: Human
Recombinant full length protein corresponding to Human Interferon gamma. Mature Interferon gamma is from aa 24-161 of SwissProt ID Q14609. aa 1-23 represents the signal peptide.
Database link: Q14609
WB: Recombinant human Interferon gamma protein (ab9659). Sandwich ELISA: Recombinant human Interferon gamma protein (ab9659).
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab231036.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
pH: 7.2
Constituent: 100% PBS
No preservative, sterile filtered
Protein A purified
affinity chromatography employing immobilized Human IFN-gamma matrix.
多克隆
IgG
unknown
Abpromise™承诺保证使用ab9657于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Detects IFN-gamma by sandwich ELISA using 100µl/well antibody solution. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIFN-gamma. Can be paired for ELISA with ab9658. Read More | |
| WB | (4) | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More |
Entrez Gene: 3458 Human
Omim: 147570 Human
SwissProt: P01579 Human
Unigene: 856 Human
ifn g antibody
IFN gamma antibody
IFN immune antibody
IFN, immune antibody
IFN-gamma antibody
IFNG antibody
IFNG_HUMAN antibody
Immune interferon antibody
Interferon gamma antibody
Type II Interferon antibody
IF 1 antibody
IFG antibody
IFI antibody
Western blot - Anti-Interferon gamma antibody (ab9657)
Lanes 2-12 : Anti-Interferon gamma antibody (ab9657) at 0.1 µg/ml
Lane 1 : Marker
Lanes 2-12 : Recombinant human Interferon gamma protein (Active) (ab9659)
Performed under non-reducing conditions.
Predicted band size: 17 kDa
Sandwich ELISA - Anti-Interferon gamma antibody (ab9657)
To detect Human IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with a compatible secondary detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-γ.
