Anti-PUMA抗体
参阅全部 PUMA 一抗
兔多克隆抗体to PUMA
Rabbit
适用于: WB, ICC/IF, IHC-Pmore details
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide:
ARARQEGSSPEPVEG
, corresponding to amino acids 2-16 of Human PUMA. (Peptide available as ab9646).
WB: K562 lysate IHC-P: Human breast carcinoma sections ICC/IF: K562 cells
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.02% Sodium azide
Immunogen affinity purified
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
多克隆
IgG
Abpromise™承诺保证使用ab9645于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 kDa. |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use a concentration of 10 µg/ml. |
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Unigene: 7660 Mouse
Unigene: 25176 Rat
Entrez Gene: 27113 Human
Entrez Gene: 170770 Mouse
Omim: 605854 Human
SwissProt: Q9BXH1 Human
SwissProt: Q99ML1 Mouse
Unigene: 467020 Human
BBC 3 antibody
Bbc3 antibody
BBC3_HUMAN antibody
BCL 2 binding component 3 antibody
Bcl-2-binding component 3 antibody
BCL2 binding component 3 antibody
JFY 1 antibody
JFY-1 antibody
JFY1 antibody
p53 up regulated modulator of apoptosis antibody
p53 up-regulated modulator of apoptosis antibody
p53 Upregulated Modulator of Apoptosis antibody
PUMA alpha antibody
PUMA/JFY1 antibody
Western blot - Anti-PUMA antibody (ab9645)
Anti-PUMA antibody (ab9645) at 2 µg/ml + K562 cell lysates at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Primary incubation: 1 hour at room temperature in 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (ab9645)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling PUMA with ab9645 at 10 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (ab9645)
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling PUMA with ab9645 at 2.5 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9645)
Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9645 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9645)
Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9645 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells.
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