Anti-hCG beta antibody [5H4-E2]

• 产品名称

  Anti-hCG beta抗体[5H4-E2]
  参阅全部 hCG beta 一抗

• 描述

  小鼠单克隆抗体[5H4-E2] to hCG beta

• 宿主

  Mouse

• 特异性

  The antibody recognizes the whole hCG beta and the C-terminal end part of hCG beta, as well as the hCG dimer molecule. It does not react with hCG alpha, FSH, LH, TSH and albumins (evaluated by simple-binding ELISA using 1.00 µg/well purified antigens (WHO standards) and 1.00 µg/ml primary antibody concentrations). This antibody labels the cytoplasm of hCG beta producing (trophoblastic) cells in placenta, mola hydatidosa, choriocarcinoma, embryonal carcinoma and some undifferenciated lung cancers, as well as, the JAR choriocarcinoma cell line. It does not react with cells of normal endometrial, testicular tissues and lung (tested by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method).

• 经测试应用

  适用于: IHC-Pmore details

• 种属反应性

  与反应: Human
  不与反应: Horse

• 免疫原

  Full length native protein (purified) corresponding to Human hCG beta.
  Database link: P01233

• 阳性对照

• ICC/IF: Human chorionic villus cells. IHC-P: Human fetal chorionic villus tissue.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.097% Sodium azide
  Constituents: Tris HCl, 0.2% BSA

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  5H4-E2

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab9582于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 114336 Human

• Omim: 118860 Human

• Omim: 608824 Human

• SwissProt: P01233 Human

• SwissProt: P0DN86 Human

• SwissProt: P0DN87 Human

• SwissProt: Q6NT52 Human

• 别名

• CGB3 antibody

• CGB3_HUMAN antibody

• CGB5 antibody

• CGB7 antibody

• CGB7_HUMAN antibody

• CGB8 antibody

• CGHB_HUMAN antibody

• Choriogonadotropin subunit beta 3 antibody

• Choriogonadotropin subunit beta 7 antibody

• Choriogonadotropin subunit beta antibody

• Chorionic gonadotropin beta polypeptide antibody

• Chorionic gonadotropin chain beta antibody

• CG beta antibody

• CG-beta antibody

• CgB antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (ab9582)

  Formalin-fixed, paraffin-embedded human seminoma tissue stained for hCG beta with ab9582 at a 1/50 dilution in immunohistochemical analysis.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (ab9582)Image from Spitalieri P et al, Cloning Stem Cells. 2009 Dec;11(4):535-56, Fig 1, DOI: 10.1089/clo.2009.0046.

  ab9582 staining hCG beta in human fetal chorionic villus by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Samples were fixed in 4% paraformaldehyde and then incubated with ab9582 in PBT [3% bovine serum albumin (BSA) and 0.5% Triton X-100 in phosphate-buffered saline (PBS)] overnight at 4°C. After extensive washing with PBT, samples were incubated with a secondary antibody conjugated to Alexa-Fluor® 488 for 1 hour at room temperature in the dark. After nuclear staining with Hoechst slides were mounted with PBS/ glycerol.