Anti-TNF alpha antibody [P/T2]

• 产品名称

  Anti-TNF alpha抗体[P/T2]
  参阅全部 TNF alpha 一抗

• 描述

  小鼠单克隆抗体[P/T2] to TNF alpha

• 宿主

  Mouse

• 特异性

  The antibody labels the cytoplasm of TNF-alpha secreting cells including macrophages.

• 经测试应用

  适用于: Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant fragment corresponding to Human TNF alpha aa 115-130.
  Database link: P01375

• 阳性对照

• Granulomatous inflammation

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.097% Sodium azide
  Constituent: 0.2% BSA

• 浓度

• 100 µg 浓度为 0.2 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  P/T2

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab9579于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7124 Human

• Omim: 191160 Human

• SwissProt: P01375 Human

• Unigene: 241570 Human

• 别名

• DIF antibody

• Differentiation inducing factor antibody

• Macrophage cytotoxic factor antibody

• Tnf antibody

• TNF superfamily member 2 antibody

• TNF superfamily, member 2 antibody

• TNF, macrophage derived antibody

• TNF, monocyte derived antibody

• TNF-a antibody

• TNF-alpha antibody

• TNFA antibody

• TNFA_HUMAN antibody

• TNFSF2 antibody

• Tumor necrosis factor (TNF superfamily member 2) antibody

• Tumor necrosis factor alpha antibody

• Tumor necrosis factor antibody

• Tumor necrosis factor ligand superfamily member 2 antibody

• Tumor Necrosis Factor, Membrane Form antibody

• Tumor necrosis factor, soluble form antibody

• APC1 antibody

• APC1 protein antibody

• Cachectin antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [P/T2] (ab9579)

  The antibody ab9579 labels the cytoplasm of TNF-a secreting cells including histiocytes/macrophages.

• 

  Flow Cytometry - Anti-TNF alpha antibody [P/T2] (ab9579)

  Overlay histogram showing THP1 cells stained with ab9579 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9579, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [P/T2] (ab9579)

  Immunohistochemisty of Human adenocarcinoma uterum (with positive reactive macrophages).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [P/T2] (ab9579)

  Immunochistochemistry of Human mandibular cyst (granulomatous inflammation with positive macrophages).