Anti-TGF alpha antibody [P/T1]

• 产品名称

  Anti-TGF alpha抗体[P/T1]
  参阅全部 TGF alpha 一抗

• 描述

  小鼠单克隆抗体[P/T1] to TGF alpha

• 宿主

  Mouse

• 特异性

  This antibody was selected and evaluated immunoserologically for recombinant human TGF-alpha and synthetic (uncoupled) peptides using simple binding and competitive ELISA. Immunohistochemical characterization of the anti-TGF-alpha monoclonal antibody was made on different human carcinomas. Recommended for immunohistochemical detection of TGF-alpha production in human tumor cells, immunoserological monitoring of progression of malignant tumours and for screening of therapeutic efficiency of different human tumors.

• 经测试应用

  适用于: IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide fragment (32-40 amino acids sequence of 50 amino acids) of transforming growth factor alpha (TGF-alpha).

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.097% Sodium azide
  Constituents: Tris HCl, 0.2% BSA

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  P/T1

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab9578于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7039 Human

• Omim: 190170 Human

• SwissProt: P01135 Human

• Unigene: 170009 Human

• 别名

• TFGA antibody

• TGF A antibody

• TGF type 1 antibody

• TGF-alpha antibody

• Tgfa antibody

• TGFA_HUMAN antibody

• Transforming growth factor alpha antibody

• Transforming growth factor alpha precursor antibody

• Wa1 antibody

• Waved 1 antibody

• EGF like TGF antibody

• EGF-like TGF antibody

• ETGF antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [P/T1] (ab9578)

  Immunohistochemical characterization of the anti-TGF-a monoclonal antibody ab9578 on human melanoma.

• 

  Flow Cytometry - Anti-TGF alpha antibody [P/T1] (ab9578)

  Overlay histogram showing A431 cells stained with ab9578 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.