Anti-CUG-BP1 antibody [3B1]

• 产品名称

  Anti-CUG-BP1抗体[3B1]
  参阅全部 CUG-BP1 一抗

• 描述

  小鼠单克隆抗体[3B1] to CUG-BP1

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, WB, ICCmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Rabbit, Cow, Pig

• 免疫原

  Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: HeLa, HEK-293T and SHSY-5Y cell lysates; Human kidney and brain tissue lysates. ICC: HeLa cells. IHC: Human Breast cancer tissue

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 10 µg 浓度为 0.8 mg/ml

• 批次浓度范围 100 µg 浓度为 0.8 - 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  3B1

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab9549于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 10658 Human

• Entrez Gene: 13046 Mouse

• Entrez Gene: 362160 Rat

• Omim: 601074 Human

• SwissProt: Q92879 Human

• SwissProt: P28659 Mouse

• SwissProt: Q4QQT3 Rat

• Unigene: 595333 Human

• Unigene: 29495 Mouse

• Unigene: 393354 Mouse

• Unigene: 22432 Rat

别名

• 50 kDa Nuclear polyadenylated RNA binding protein antibody

• 50 kDa nuclear polyadenylated RNA-binding protein antibody

• Bruno like 2 antibody

• bruno like protein 2 antibody

• Bruno-like protein 2 antibody

• BRUNOL 2 antibody

• BRUNOL2 antibody

• CELF 1 antibody

• CELF-1 antibody

• celf1 antibody

• CELF1 CUGBP, Elav like family member 1 antibody

• CELF1_HUMAN antibody

• CUG BP and ETR 3 like factor 1 antibody

• CUG BP antibody

• CUG BP1 antibody

• CUG RNA binding protein antibody

• CUG triplet repeat RNA binding protein 1 antibody

• CUG triplet repeat RNA-binding protein 1 antibody

• CUG-BP antibody

• CUG-BP- and ETR-3-like factor 1 antibody

• CUG-BP1 antibody

• CUGBP 1 antibody

• CUGBP and ETR3 like factor 1 antibody

• CUGBP antibody

• CUGBP Elav like family member 1 antibody

• CUGBP Elav-like family member 1 antibody

• CUGBP1 antibody

• Cytidine uridine guanosine binding protein 1 antibody

• Deadenylation factor CUG BP antibody

• Deadenylation factor CUG-BP antibody

• Deadenylation factor CUGBP antibody

• EDEN BP antibody

• EDEN BP homolog antibody

• EDEN-BP antibody

• EDEN-BP homolog antibody

• embryo deadenylation element binding protein antibody

• embryo deadenylation element binding protein homolog antibody

• Embryo deadenylation element-binding protein homolog antibody

• hNab 50 antibody

• hNab50 antibody

• NAB 50 antibody

• NAB50 antibody

• NAPOR antibody

• Nuclear polyadenylated RNA binding protein 50 kD antibody

• Nuclear polyadenylated RNA binding protein antibody

• RNA binding protein BRUNOL 2 antibody

• RNA binding protein BRUNOL2 antibody

• RNA-binding protein BRUNOL-2 antibody

• 

  Immunocytochemistry - Anti-CUG-BP1 antibody [3B1] (ab9549)

  ab9549 staining CUG-BP1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab9549 at 1 µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Also suitable in cells fixed with 100% methanol (5 min).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• 

  Western blot - Anti-CUG-BP1 antibody [3B1] (ab9549)

  All lanes : Anti-CUG-BP1 antibody [3B1] (ab9549) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
  Lane 3 : Human kidney tissue lysate - total protein (ab30203)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 54 kDa
  Observed band size: 54 kDa
  
  
  Exposure time: 20 minutes
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUG-BP1 antibody [3B1] (ab9549)

  IHC image of CUG-BP1 staining in human normal kidney formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9549, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

   

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre