HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control

• 产品名称

  HRP Anti-GAPDH抗体[mAbcam 9484] - Loading Control
  参阅全部 GAPDH 一抗

• 描述

  HRP小鼠单克隆抗体[mAbcam 9484] to GAPDH - Loading Control

• 宿主

  Mouse

• 偶联物

  HRP

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  预测可用于: Rabbit, Chicken, Cow, Dog, Xenopus laevis, Chinese hamster

• 免疫原

  Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: HeLa, HEK-293, NIH/3T3 and PC-12 whole cell lysates.

• 常规说明

  According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.

  This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

• 存储溶液

  Preservative: 0.1% 10% Proclin 300 Solution
  Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)

• 浓度

• 100 µl 浓度为 0.5 mg/ml

• 纯度

  IgG fraction

• 克隆

  单克隆

• 克隆编号

  mAbcam 9484

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG2b

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab9482于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 374193 Chicken

• Entrez Gene: 281181 Cow

• Entrez Gene: 403755 Dog

• Entrez Gene: 2597 Human

• Entrez Gene: 100042025 Mouse

• Entrez Gene: 14433 Mouse

• Entrez Gene: 100009074 Rabbit

• Entrez Gene: 24383 Rat

• Entrez Gene: 685186 Rat

• Entrez Gene: 380259 Xenopus laevis

• Omim: 138400 Human

• SwissProt: P00356 Chicken

• SwissProt: P10096 Cow

• SwissProt: Q28259 Dog

• SwissProt: P04406 Human

• SwissProt: P16858 Mouse

• SwissProt: P46406 Rabbit

• SwissProt: P04797 Rat

• SwissProt: P51469 Xenopus laevis

• Unigene: 544577 Human

• Unigene: 592355 Human

• Unigene: 598320 Human

• Unigene: 304088 Mouse

• Unigene: 309092 Mouse

• Unigene: 317779 Mouse

• Unigene: 343110 Mouse

• Unigene: 392463 Mouse

• Unigene: 392480 Mouse

• Unigene: 414470 Mouse

• Unigene: 458138 Mouse

• Unigene: 458416 Mouse

• Unigene: 475698 Mouse

• Unigene: 129558 Rat

• Unigene: 91450 Rat

• Unigene: 995 Xenopus laevis

别名

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• 

  Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)

  All lanes : HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) at 1/1000 dilution
  
  Lane 1 : HeLa Whole Cell Lysate
  Lane 2 : Hek293 Whole Cell Lysate
  Lane 3 : NIH 3T3 Whole Cel lLysate
  Lane 4 : PC12 Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 40.2 kDa
  Observed band size: 37 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 4 minutes