Anti-Sarcomeric Alpha Actinin antibody [EA-53]

• 产品名称

  Anti-Sarcomeric Alpha Actinin抗体[EA-53]
  参阅全部 Sarcomeric Alpha Actinin 一抗

• 描述

  小鼠单克隆抗体[EA-53] to Sarcomeric Alpha Actinin

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, WBmore details

• 种属反应性

  与反应: Rat, Human
  预测可用于: Rabbit

• 免疫原

  Full length native protein (purified) corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin.

• 阳性对照

• WB: Mouse and rat skeletal muscle and rat heart tissue lysates. IHC-P: Human skeletal muscle tissue.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 9th September 2019. Lot numbers higher than GR3258034 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  Storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.

• 存储溶液

  pH: 7.40
  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Proprietary Purification

• 纯化说明

  Purified from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  EA-53

• 骨髓瘤

  unknown

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9465于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 88 Human

• Entrez Gene: 291245 Rat

• Omim: 102573 Human

• SwissProt: P35609 Human

• Unigene: 498178 Human

• 别名

• ACTN2 antibody

• ACTN2_HUMAN antibody

• Alpha actinin 2 antibody

• Alpha actinin skeletal muscle antibody

• Alpha actinin skeletal muscle isoform 2 antibody

• Alpha-actinin skeletal muscle isoform 2 antibody

• Alpha-actinin-2 antibody

• CMD1AA antibody

• F actin cross linking protein antibody

• F-actin cross-linking protein antibody

• Actin binding protein antibody

• Actinin alpha 2 antibody

• ACTN 2 antibody

• 

  Western blot - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

  Lane 1 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/500 dilution
  Lane 2 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/1000 dilution
  Lane 3 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/2000 dilution
  Lane 4 : No antibody control
  
  All lanes : Rat skeletal muscle extract
  
  Secondary
  All lanes : Goat Anti-Mouse IgG (Fab) (HRP) at 1/4000 dilution
  
  Predicted band size: 103 kDa
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

  Immunohistochemistry formalin-fixed, paraffin-embedded human tongue labeling Alpha Actinin with ab9465 at 12 μg/mL.