Anti-wide spectrum Cytokeratin抗体
兔多克隆抗体to wide spectrum Cytokeratin
Rabbit
This antibody reacts with cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68 kDa.
适用于: IHC-P, ICC, ICC/IF, Flow Cyt, WB, IHC-Frmore details
与反应: Mouse, Rat, Cow, Dog, Human, Pig, Cynomolgus monkey, Common marmoset
Full length native protein (purified) corresponding to Cow wide spectrum Cytokeratin. Cytokeratin isolated from bovine muzzle epidermis.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: 10% PBS, 1% BSA
Protein A purified
多克隆
IgG
unknown
Abpromise™承诺保证使用ab9377于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (28) | Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. This antibody may be diluted to a titer of 1:75-1:200 in an ABC method. |
| ICC | (5) | Use at an assay dependent concentration. |
| ICC/IF | (4) | Use at an assay dependent concentration. |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. | |
| WB | (4) | Use at an assay dependent concentration. Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
| IHC-Fr | (2) | Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
pan cytokeratin antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)This image is courtesy of an Abreview submitted by Cameron Johnstone.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of 10% formalin-fixed Human transitional cell carcinoma of the kidney. Stained with ab9377 at 1/100 dilution. Secondary antibody used was goat anti-rabbit Alexa-568® at 1/500 dilution. Blocking was done with 5% serum for 1 hour at 25°C. The sample was incubated with the primary antibody for 1 hour at 25°C with 5% normal goat serum. Antigen retrieval method was heat mediated with EDTA pH 8.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)ab9377 staining wide spectrum Cytokeratin in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)Image is courtesy of an anonymous AbReview
ab9377 staining wide spectrum Cytokeratin in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with BlockAid for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/500 in 1% BSA + PBS) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)This image is courtesy of an Abreview by Carl Hobbs.
ab9377 staining wide spectrum Cytokeratin in rat liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)This image is courtesy of an Abreview by Carl Hobbs.
ab9377 staining wide spectrum Cytokeratin in common marmoset lung tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-wide spectrum Cytokeratin antibody (ab9377)
Ab9377 positively staining Cytokeratin (green) in paraffin fixed mouse oesophagus at 1/250.
抱歉，暂无浏览记录
