Anti-AACT抗体
参阅全部 AACT 一抗
兔多克隆抗体to AACT
Rabbit
适用于: IHC-P, Sandwich ELISA, WBmore details
与反应: Human
预测可用于: Mammals
不与反应: Mouse
Full length native protein (purified) corresponding to AACT. Alpha-1-antichymotrypsin isolated from human serum.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.3
Constituents: PBS, 0.01425% Magnesium chloride, 0.00154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.01% BSA
Protein A purified
多克隆
IgG
unknown
Abpromise™承诺保证使用ab9374于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/25 - 1/50. ABC method. | |
| Sandwich ELISA | Use at an assay dependent concentration. For sandwich ELISA, use this antibody as Detection at an assay dependent concentration with Mouse monoclonal to AACT (ab54693) as Capture. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 48 kDa). Assay Dependent |
Entrez Gene: 12 Human
Omim: 107280 Human
SwissProt: P01011 Human
Unigene: 534293 Human
Unigene: 710488 Human
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Serpin family A member 3 antibody
Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
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AACT antibody
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Western blot - Anti-AACT antibody (ab9374)
All lanes : Anti-AACT antibody (ab9374) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 48 kDa
Observed band size: 70 kDawhy is the actual band size different from the predicted?
Sandwich ELISA - Anti-AACT antibody (ab9374)
Standard Curve for AACT (Analyte: AACT protein (Human) (ab80517) ); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to AACT (ab54693) at 5µg/ml and Detector Antibody Rabbit polyclonal to AACT (ab9374) at 0.5µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody (ab9374)
Human normal skin. Staining is mainly membrane-bound and cytoplasmic. Left panel: with primary antibody at 2 ug/ml. Right panel: negative control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot - Anti-AACT antibody (ab9374)
