Anti-DDB1 antibody

• 产品名称

  Anti-DDB1抗体
  参阅全部 DDB1 一抗

• 描述

  山羊多克隆抗体to DDB1

• 宿主

  Goat

• 经测试应用

  适用于: WB, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Rat, Cow, Dog

• 免疫原

  Synthetic peptide corresponding to Human DDB1 aa 1128-1140 (C terminal).
  Sequence:

  C-DLIKVVEELTRIH

  
  (Peptide available as ab23152)

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: HeLa lysate, HepG2 lysate, Jurkat lysate, NIH3T3 lysate and NSO lysate. IHC-P: Human cortex tissue.

• 常规说明

  
  

  Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: Tris buffered saline, 0.5% BSA

• 浓度

• 100 µg 浓度为 0.5 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

• Primary antibody说明

  Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab9194于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 511951 Cow

• Entrez Gene: 100290337 Human

• Entrez Gene: 1642 Human

• Entrez Gene: 13194 Mouse

• Entrez Gene: 64470 Rat

• Omim: 600045 Human

• SwissProt: A1A4K3 Cow

• SwissProt: Q16531 Human

• SwissProt: Q3U1J4 Mouse

• SwissProt: Q9ESW0 Rat

• Unigene: 290758 Human

• Unigene: 289915 Mouse

• Unigene: 466856 Mouse

• Unigene: 8402 Rat

别名

• Damage specific DNA binding protein 1 antibody

• Damage-specific DNA-binding protein 1 antibody

• DDB 1 antibody

• DDB p127 subunit antibody

• Ddb1 antibody

• DDB1_HUMAN antibody

• DDBa antibody

• DNA damage binding protein 1 antibody

• DNA damage-binding protein 1 antibody

• DNA damage-binding protein a antibody

• HBV X-associated protein 1 antibody

• UV damaged DNA binding factor antibody

• UV damaged DNA binding protein 1 antibody

• UV DDB 1 antibody

• UV DDB1 antibody

• UV-damaged DNA-binding factor antibody

• UV-damaged DNA-binding protein 1 antibody

• UV-DDB 1 antibody

• X associated protein 1 antibody

• XAP 1 antibody

• XAP-1 antibody

• XAP1 antibody

• Xeroderma pigmentosum group E complementing protein antibody

• Xeroderma pigmentosum group E-complementing protein antibody

• XPCe antibody

• XPE antibody

• XPE BF antibody

• XPE binding factor antibody

• XPE-BF antibody

• XPE-binding factor antibody

• 

  Western blot - Anti-DDB1 antibody (ab9194)

  All lanes : Anti-DDB1 antibody (ab9194) at 1 µg/ml
  
  Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
  Lane 2 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
  Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
  
  Lysates/proteins at 35 µg per lane.
  
  Predicted band size: 127 kDa
  Observed band size: 140 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Primary incubation for 1 hour. Detected by chemiluminescence. RIPA buffer used.

• 

  Western blot - Anti-DDB1 antibody (ab9194)

  All lanes : Anti-DDB1 antibody (ab9194) at 0.01 µg/ml
  
  Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
  Lane 2 : NSO (Murine myeloma cells) whole cell lysate
  
  Lysates/proteins at 35 µg per lane.
  
  Predicted band size: 127 kDa
  Observed band size: 140,150 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Primary incubation for 1 hour. Detected by chemiluminescence. RIPA buffer used.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab9194)

  Left panel: Immunohistochemical analysis of paraffin-embedded human cortex tissue labelling DDB1 with ab9194 at 4µg/ml. Heat mediated antigen retrieval with citrate buffer pH 6, HRP-staining.

  Right panel: Immunohistochemical analysis of paraffin-embedded human cortex tissue with no primary antibody.