Anti-HA tag抗体
参阅全部 HA tag 一抗
鸡多克隆抗体to HA tag
Chicken
ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate.
适用于: WB, ELISA, ICC/IFmore details
与反应: Species independent
Synthetic peptide corresponding to Influenza A HA tag conjugated to keyhole limpet haemocyanin. Influenza hemagglutinin-HA (epitope)
ICC/IF: CHO cells transfected with 12 tags constructs (CHO-12 tags) and transfected with GFP-HA constructs (CHO-GFP-HA).
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Preservative: 0.1% Sodium azide
浓度
250 µg 浓度为 1 mg/ml
IgY fraction
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
多克隆
unknown
unknown
Abpromise™承诺保证使用ab9111于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 4 - 5 µg/ml. |
HA epitope tag antibody
HA1 antibody
HA2 antibody
hemagglutinin antibody
Hemagglutinin HA1 chain antibody
Hemagglutinin HA2 chain antibody
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)
Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilised with 0.1% Triton X-100 in cells transfected with GFP-HA contrsucts (as CHO-GFP-HA) or parental non- transfected CHO cells labelling with chicken anti-HA tag (ab9111) at 5µg/ml, followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5µg/ml (Red). Nucleus was counterstained with DAPI (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)
Immunofluorescent analysis of 4% paraformaldehyde (PFA) -fixed, permeabilized with 0.1% Triton X-100 CHO cells transfected with 12 tags constructs (CHO-12 tags) labelling HA tag with ab9111 at 1/250 dilution (4μg/mL), followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 1/500 dilution (2 μg/mL) (Red). Nucleus was counterstained with DAPI (white). Bottom panel images showed Parallel staining in parental CHO cell line.
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)
Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton X-100 CHO cells transfected with GFP-HA constructs (CHO-GFP-HA) labelling HA tag with ab9111 at 5μg/mL, followed by Goat Anti- Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5 μg/mL (Red). Nucleus was counterstained with DAPI (Blue).
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