Anti-HLA Class I antibody [MEM-147]

• 产品名称

  Anti-HLA Class I抗体[MEM-147]
  参阅全部 HLA Class I 一抗

• 描述

  小鼠单克隆抗体[MEM-147] to HLA Class I

• 宿主

  Mouse

• 特异性

  Reacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). Positive in Western blotting (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32 (ab7855).

• 经测试应用

  适用于: Flow Cyt, WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to HLA Class I. PHA-activated peripheral blood lymphocytes

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from TCS. Purity >95% by SDS-PAGE.

• 克隆

  单克隆

• 克隆编号

  MEM-147

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab9090于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 3105 Human

• Entrez Gene: 3133 Human

• Entrez Gene: 3134 Human

• Entrez Gene: 3135 Human

• Omim: 142800 Human

• SwissProt: O15506 Human

• SwissProt: P04439 Human

• SwissProt: P13747 Human

• SwissProt: Q5MCQ6 Human

• SwissProt: Q5ZGM8 Human

• SwissProt: Q7YQ33 Human

• SwissProt: Q861B7 Human

• SwissProt: Q8MHP7 Human

• SwissProt: Q9MY64 Human

• SwissProt: Q9MY72 Human

• SwissProt: Q9MYB7 Human

别名

• DKFZp686P19218 antibody

• EA1.2 antibody

• EA2.1 antibody

• HLA 5.4 antibody

• HLA 6.2 antibody

• HLA A antibody

• HLA B antibody

• HLA C antibody

• HLA CDA12 antibody

• HLA E antibody

• HLA F antibody

• HLA G antibody

• HLA G histocompatibility antigen class I G antibody

• HLAF antibody

• Major histocompatibility complex class I A antibody

• Major histocompatibility complex class I antibody

• Major histocompatibility complex class I E antibody

• Major histocompatibility complex class I F antibody

• MHC antibody

• MHC Class I antigen antibody

• MHC G antibody

• 

  Flow Cytometry - Anti-HLA Class I antibody [MEM-147] (ab9090)

  Flow cytometric analysis of human leukocytes stained using ab9090 (concentration in sample 1.67 μg/ml, GAM PE, red-filled) from human leukocytes unstained by primary antibody (GAM PE, black-dashed).

• 

  Western blot - Anti-HLA Class I antibody [MEM-147] (ab9090)

  Anti-HLA Class I antibody [MEM-147] (ab9090) at 1/100 dilution + Human Ramos B cell line lysates (1% laurylmaltoside)
  
  Secondary
  Goat anti-mouse IgG (H+L)-HRP conjugate
  
  Performed under non-reducing conditions.
  

• 

  Flow Cytometry - Anti-HLA Class I antibody [MEM-147] (ab9090)

  Overlay histogram showing Jurkat cells stained with ab9090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9090, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.