Anti-Histone H4 (di methyl K20)抗体
参阅全部 Histone H4 一抗
兔多克隆抗体to Histone H4 (di methyl K20)
Rabbit
适用于: IP, WB, ChIP, ICC/IF, IHC-Pmore details
与反应: Mouse, Cow, Human, Drosophila melanogaster, Schizosaccharomyces pombe, Toxoplasma gondii
预测可用于: Rat, a wide range of other species
不与反应: Saccharomyces cerevisiae
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
WB: Calf thymus histone preparation and HeLa whole cell extract and S. pombe. IHC-P: FFPE human pancreas adenocarcinoma tissue. ICC/IF: HeLa cell line
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab9052于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | (5) | 1/2000. Detects a band of approximately 11 kDa. |
| ChIP | (1) | Use at an assay dependent concentration. PubMed: 20829797 |
| ICC/IF | (2) | Use a concentration of 1 µg/ml. |
| IHC-P | (2) | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Entrez Gene: 318846 Drosophila melanogaster
Entrez Gene: 3771854 Drosophila melanogaster
Entrez Gene: 3771893 Drosophila melanogaster
Entrez Gene: 3771908 Drosophila melanogaster
Entrez Gene: 3771935 Drosophila melanogaster
Entrez Gene: 3771938 Drosophila melanogaster
Entrez Gene: 3771941 Drosophila melanogaster
Entrez Gene: 3771947 Drosophila melanogaster
Entrez Gene: 3772096 Drosophila melanogaster
Entrez Gene: 3772113 Drosophila melanogaster
Entrez Gene: 3772129 Drosophila melanogaster
Entrez Gene: 3772172 Drosophila melanogaster
Entrez Gene: 3772211 Drosophila melanogaster
Entrez Gene: 3772254 Drosophila melanogaster
Entrez Gene: 3772314 Drosophila melanogaster
Entrez Gene: 3772317 Drosophila melanogaster
Entrez Gene: 3772319 Drosophila melanogaster
Entrez Gene: 3772325 Drosophila melanogaster
Entrez Gene: 3772509 Drosophila melanogaster
Entrez Gene: 3772519 Drosophila melanogaster
Entrez Gene: 3772666 Drosophila melanogaster
Entrez Gene: 3772708 Drosophila melanogaster
Entrez Gene: 41773 Drosophila melanogaster
Entrez Gene: 121504 Human
Entrez Gene: 554313 Human
Entrez Gene: 8294 Human
Entrez Gene: 8359 Human
Entrez Gene: 8360 Human
Entrez Gene: 8361 Human
Entrez Gene: 8362 Human
Entrez Gene: 8363 Human
Entrez Gene: 8364 Human
Entrez Gene: 8365 Human
Entrez Gene: 8366 Human
Entrez Gene: 8367 Human
Entrez Gene: 8368 Human
Entrez Gene: 8370 Human
Entrez Gene: 100041230 Mouse
Entrez Gene: 319155 Mouse
Entrez Gene: 319156 Mouse
Entrez Gene: 319157 Mouse
Entrez Gene: 319158 Mouse
Entrez Gene: 319159 Mouse
Entrez Gene: 319160 Mouse
Entrez Gene: 319161 Mouse
Entrez Gene: 320332 Mouse
Entrez Gene: 326619 Mouse
Entrez Gene: 326620 Mouse
Entrez Gene: 674678 Mouse
Entrez Gene: 69386 Mouse
Entrez Gene: 97122 Mouse
Omim: 142750 Human
Omim: 602822 Human
Omim: 602823 Human
Omim: 602824 Human
Omim: 602825 Human
Omim: 602826 Human
Omim: 602827 Human
Omim: 602828 Human
Omim: 602829 Human
Omim: 602830 Human
Omim: 602831 Human
Omim: 602833 Human
SwissProt: P84040 Drosophila melanogaster
SwissProt: P62805 Human
SwissProt: P62806 Mouse
Unigene: 21500 Drosophila melanogaster
Unigene: 29514 Drosophila melanogaster
Unigene: 29527 Drosophila melanogaster
Unigene: 30219 Drosophila melanogaster
Unigene: 30220 Drosophila melanogaster
Unigene: 30221 Drosophila melanogaster
Unigene: 30223 Drosophila melanogaster
Unigene: 30868 Drosophila melanogaster
Unigene: 30869 Drosophila melanogaster
Unigene: 30871 Drosophila melanogaster
Unigene: 30872 Drosophila melanogaster
Unigene: 30873 Drosophila melanogaster
Unigene: 30876 Drosophila melanogaster
Unigene: 33873 Drosophila melanogaster
Unigene: 5747 Drosophila melanogaster
Unigene: 143080 Human
Unigene: 247816 Human
Unigene: 248172 Human
Unigene: 248178 Human
Unigene: 248179 Human
Unigene: 278483 Human
Unigene: 46423 Human
Unigene: 528055 Human
Unigene: 533295 Human
Unigene: 55468 Human
Unigene: 591790 Human
Unigene: 655235 Human
Unigene: 662174 Human
Unigene: 706635 Human
Unigene: 718935 Human
Unigene: 144300 Mouse
Unigene: 14775 Mouse
Unigene: 158272 Mouse
Unigene: 227295 Mouse
Unigene: 228709 Mouse
Unigene: 246720 Mouse
Unigene: 255646 Mouse
Unigene: 260530 Mouse
Unigene: 261642 Mouse
Unigene: 261662 Mouse
Unigene: 261664 Mouse
Unigene: 377875 Mouse
Unigene: 442307 Mouse
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FO108 antibody
H4 antibody
H4.k antibody
H4/a antibody
H4/b antibody
H4/c antibody
H4/d antibody
H4/e antibody
H4/g antibody
H4/h antibody
H4/I antibody
H4/j antibody
H4/k antibody
H4/m antibody
H4/n antibody
H4/p antibody
H4_HUMAN antibody
H4F2 antibody
H4F2iii antibody
H4F2iv antibody
H4FA antibody
H4FB antibody
H4FC antibody
H4FD antibody
H4FE antibody
H4FG antibody
H4FH antibody
H4FI antibody
H4FJ antibody
H4FK antibody
H4FM antibody
H4FN antibody
H4M antibody
HIST1H4A antibody
HIST1H4B antibody
HIST1H4C antibody
HIST1H4D antibody
HIST1H4E antibody
HIST1H4F antibody
HIST1H4H antibody
HIST1H4I antibody
HIST1H4J antibody
HIST1H4K antibody
HIST1H4L antibody
HIST2H4 antibody
HIST2H4A antibody
Hist4h4 antibody
Histone 1 H4a antibody
Histone 1 H4b antibody
Histone 1 H4c antibody
Histone 1 H4d antibody
Histone 1 H4e antibody
Histone 1 H4f antibody
Histone 1 H4h antibody
Histone 1 H4i antibody
Histone 1 H4j antibody
Histone 1 H4k antibody
Histone 1 H4l antibody
Histone 2 H4a antibody
histone 4 H4 antibody
Histone H4 antibody
MGC24116 antibody
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (di methyl K20) antibody (ab9052)
ab9052 staining Histone H4 (di methyl K20) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab9052 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunoprecipitation - Anti-Histone H4 (di methyl K20) antibody (ab9052)
Histone H4 (di methyl K20) was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Rabbit polyclonal to Histone H4 (di methyl K20) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab9052.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 14kDa: Histone H4 (di methyl K20).
Western blot - Anti-Histone H4 (di methyl K20) antibody (ab9052)This image is courtesy of Steve Sanders, Tony Kouzarides lab, University of Cambridge
All lanes : Anti-Histone H4 (di methyl K20) antibody (ab9052) at 1/2000 dilution
Lane 1 : yH4 17-24 peptide
Lane 2 : Unmodified yH4 17-24 peptide
Lane 3 : Mono methyl K20 yH4 17-24 peptide
Lane 4 : Di methyl K20 yH4 17-24 peptide
Lane 5 : Tri methyl K20 yH4 17-24 peptide
Blocking peptides at 1 µg/ml per lane.
Performed under reducing conditions.
Western blot - Anti-Histone H4 (di methyl K20) antibody (ab9052)
All lanes : Upper blot - Histone H4 antibody (gift of A. Verreault)
Lower blot - Rabbit polyclonal to Histone H4 di methyl K20 (ab9052) at 1/2000
Lane 1 : S.cerevisiae extract
Lane 2 : S.pombe extract
Lane 3 : S.pombe rH4
Lane 4 : Drosophila rH4
Lane 5 : Histones (Roche)
Di methylation at K20 is seen in S. pombe and in calf thymus (Roche). S. cerevisiae lacks K20 (di) methylation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (di methyl K20) antibody (ab9052)
IHC image of ab9052 staining in human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9052, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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