Anti-68kDa Neurofilament/NF-L抗体
参阅全部 68kDa Neurofilament/NF-L 一抗
兔多克隆抗体to 68kDa Neurofilament/NF-L
Rabbit
Specifically recognizes the light neurofilament subunit (~68 kDa).
适用于: WB, ICC/IF, IHC-FrFlmore details
与反应: Mouse, Rat
预测可用于: Bird, Mammals
Full length native protein (purified) corresponding to Pig 68kDa Neurofilament/NF-L. prepared from spinal cords by the method of Delacourte et al. and this cytoskeletal material was dissolved in 6M urea. Purified by ion exchange chromatography, then preparative gel electrophoresis.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab9035于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/5000. | |
| ICC/IF | (1) | 1/500. |
| IHC-FrFl | 1/5000. |
Entrez Gene: 18039 Mouse
SwissProt: P08551 Mouse
Unigene: 1956 Mouse
Unigene: 18568 Rat
CMT1F antibody
CMT2E antibody
FLJ53642 antibody
Light molecular weight neurofilament protein antibody
NEFL antibody
Neurofilament light antibody
Neurofilament light polypeptide 68kDa antibody
Neurofilament light polypeptide antibody
Neurofilament protein, light chain antibody
Neurofilament subunit NF L antibody
Neurofilament triplet L protein antibody
NF-L antibody
NF68 antibody
NFL antibody
NFL_HUMAN antibody
68 kDa neurofilament protein antibody
68kDa Neurofilament antibody
68kDa neurofilament protein antibody
Western blot - Anti-68kDa Neurofilament/NF-L antibody (ab9035)
Lane 1 : Protein ladder
Lanes 2-5 : Anti-68kDa Neurofilament/NF-L antibody (ab9035) at 1/20000 dilution
Lane 2 : Rat brain
Lane 3 : Rat spinal cord
Lane 4 : Mouse brain
Lane 5 : Mouse spinal cord
Observed band size: 68 kDawhy is the actual band size different from the predicted?
Immunohistochemistry - Free Floating - Anti-68kDa Neurofilament/NF-L antibody (ab9035)
Immunohistochemistry (Free Floating) analysis of mouse cerebellum staining 68kDa Neurofilament/NF-L with ab9035 (1/5000) in red. Costained with chicken pAb to MBP (1/5000) in green and DAPI in blue. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.
Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (ab9035)
ab9035 staining anti 68kDa Neurofilament/NF-L in mixed neuron/glia cultures from newborn rat brain by ICC/IF (Immunocytochemistry/immunofluorescence). Samples were incubated with primary antibody (1/500) (red) and co-stained with ab4573 for anti Peripherin (green).
Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (ab9035)This image is courtesy of an Abreview submitted by Dr Luis Craveiro
ab9035 at a 1/300 dilution staining rat hippocampal organotypic slice cultures by ICC/IF. The primary antibody was incubated with the cells for 120 hours (this time allows for the antibody to penetrate a layer of glial cells that grows while the slice is in culture). Bound antibody is detected using a Cy3 conjugated Indocarbocyanine.
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