Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker

• 产品名称

  Anti-Cytokeratin 10抗体[DE-K10] - Cytoskeleton Marker
  参阅全部 Cytokeratin 10 一抗

• 描述

  小鼠单克隆抗体[DE-K10] to Cytokeratin 10 - Cytoskeleton Marker

• 宿主

  Mouse

• 特异性

  Reacts with keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.

• 经测试应用

  适用于: IHC-Fr, IHC-Pmore details

• 种属反应性

  与反应: Dog, Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human Cytokeratin 10. Cytoskeletal preparation extracted from human epidermis

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  DE-K10

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab9026于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 491006 Dog

• Entrez Gene: 3858 Human

• Omim: 148080 Human

• SwissProt: Q6EIZ0 Dog

• SwissProt: P13645 Human

• Unigene: 99936 Human

• 别名

• CK-10 antibody

• Cytokeratin-10 antibody

• EHK antibody

• K10 antibody

• K1C10_HUMAN antibody

• Keratin 10 antibody

• Keratin 10 type I antibody

• Keratin antibody

• Keratin type i cytoskeletal 10 antibody

• Keratin type I cytoskeletal 59 kDa antibody

• Keratin-10 antibody

• Keratin10 antibody

• KPP antibody

• KRT10 antibody

• type I cytoskeletal 10 antibody

• BCIE antibody

• BIE antibody

• CK 10 antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)

  IHC image on a frozen section of  dog skin showing its strong reactivity in the keratinizing epidermal cells.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)

  IHC image of ab9026 staining in Human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9026, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [DE-K10] - Cytoskeleton Marker (ab9026)Image from Reinheckel T et al, J Cell Sci. 2005 Aug 1;118(Pt 15):3387-95, Fig 3. DOI 10.1242/?jcs.02469

  Mesenchymal-epidermal interactions assessed by heterologous organotypic co-cultures (OTC). Heterologous OTC consisting of mice deficient for cathepsin L +/+ fibroblasts in collagen type I gels topped by normal human primary keratinocytes were grown air-exposed for 7 days. Paraffin sections were stained in haematoxylin and eosin (HE) or by immunohistochemistry for the proliferation marker Ki67 (Ki67, brown nuclear staining) and the differentiation markers cytokeratin 10 (K10, ab9026 1/100 dilution, brown staining) and transglutaminase (TG, brown staining). Bar 100 µm.