Anti-Cytokeratin 5 + 8 antibody [RCK102]

• 产品名称

  Anti-Cytokeratin 5 + 8抗体[RCK102]

• 描述

  小鼠单克隆抗体[RCK102] to Cytokeratin 5 + 8

• 宿主

  Mouse

• 特异性

  This antibody reacts with cytokeratin 5 and cytokeratin 8, recognizing virtually all epithelial tissues and carcinomas.

• 经测试应用

  适用于: Flow Cyt, IHC-Fr, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human Cytokeratin 5 + 8. Human lung cancer cell line (MR21).

• 常规说明

  
  

  Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• Primary antibody说明

  Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

• 克隆

  单克隆

• 克隆编号

  RCK102

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab9005于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3852 Human

• Entrez Gene: 3856 Human

• Omim: 148040 Human

• Omim: 148060 Human

• SwissProt: P05787 Human

• SwissProt: P13647 Human

• 别名

• CYK8 antibody

• Cytokeratin 5 antibody

• Cytokeratin 8 antibody

• K5 antibody

• K8 antibody

• Keratin 5 antibody

• Keratin 8 antibody

• Keratin type II cytoskeletal 5 antibody

• Keratin type II cytoskeletal 8 antibody

• KRT5 antibody

• KRT8 antibody

• CK5 antibody

• CK8 antibody

• CYK 8 antibody

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (ab9005)

  IHC image of ab9005 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9005, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

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  Flow Cytometry - Anti-Cytokeratin 5 + 8 antibody [RCK102] (ab9005)

  Overlay histogram showing A431 cells stained with ab9005 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9005, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
  
  

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  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (ab9005)

  Immunohistochemistry analysis on frozen sections of human liver hepatocytes labeling Cytokeratin 5 + 8 using ab9005.

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  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (ab9005)

  Immunohistochemistry analysis on frozen sections of human liver hepatocytes and bile ducts labeling Cytokeratin 5 + 8 using ab9005.

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  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (ab9005)

  Immunohistochemistry analysis on frozen sections of human urinary bladder epithelium labeling Cytokeratin 5 + 8 using ab9005.