Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker

• 产品名称

  Anti-Lamin A + Lamin C抗体[131C3] -核Envelope Marker
  参阅全部 Lamin A + Lamin C 一抗

• 描述

  小鼠单克隆抗体[131C3] to Lamin A + Lamin C -核Envelope Marker

• 宿主

  Mouse

• 特异性

  ab8984 reacts with lamins A and C. Lamins do not appear to be universally distributed among different cell and tissue types. ab8984 has been shown to react with HT1080 cells in Western blot. Other cell/tissue types have not been tested. Customer feedback seems to indicate that ab8984 does not detect Lamin A+C in rat brain lysates (please see AbReviews).

• 经测试应用

  适用于: Flow Cyt, IHC-Frmore details

• 种属反应性

  与反应: Human, Pig
  

• 免疫原

  Full length native protein (purified) corresponding to Rat Lamin A + Lamin C aa 300-600. Immunogen is purified rat liver lamins.
  Database link: P02545

• 表位

  Between residues 319-566.

• 阳性对照

• IHC-Fr: Human colon tissue; Pig skin tissue Flow Cyt: HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: 99% PBS

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  131C3

• 骨髓瘤

  P3x63-Ag8.653

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab8984于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4000 Human

• Entrez Gene: 100126859 Pig

• Omim: 150330 Human

• SwissProt: P02545 Human

• SwissProt: Q3ZD69 Pig

• Unigene: 594444 Human

• 别名

• CDDC antibody

• CMD1A antibody

• CMT2B1 antibody

• EMD2 antibody

• FPL antibody

• FPLD antibody

• FPLD2 antibody

• HGPS antibody

• IDC antibody

• Lamin A antibody

• Lamin A/C antibody

• Lamin A/C like 1 antibody

• Lamin antibody

• Lamin C antibody

• lamin-a antibody

• Lamin-A/C antibody

• LDP1 antibody

• LFP antibody

• LGMD1B antibody

• Limb girdle muscular dystrophy 1B (autosomal dominant) antibody

• LMN 1 antibody

• LMN A antibody

• LMN C antibody

• LMN1 antibody

• LMNA antibody

• LMNA_HUMAN antibody

• LMNC antibody

• LMNL1 antibody

• Prelamin A/C antibody

• PRO1 antibody

• Renal carcinoma antigen NY REN 32 antibody

• Renal carcinoma antigen NY-REN-32 antibody

• Renal carcinoma antigen NYREN32 antibody

• 70 kDa lamin antibody

• Cardiomyopathy dilated 1A (autosomal dominant) antibody

• CDCD1 antibody

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  Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)

  IHC-Fr of human colon showing nuclear lamina staining in epithelial and connective tissue cells.

• 

  Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)

  ab8984 (1/500) on frozen sections of pig skin showing strong positive staining in nuclei of the epidermal cells and to a lesser extent in the connective tissue.

• 

  Flow Cytometry - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)

  Overlay histogram showing HeLa cells stained with ab8984 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481)  / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8984, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984)

  ab8984 on frozen sections of human colon showing strong positive staining in nuclei of the epithelial cells and to a lesser extent in the connective tissue.