Anti-Lamin B2 antibody [LN43]

• 产品名称

  Anti-Lamin B2抗体[LN43]
  参阅全部 Lamin B2 一抗

• 描述

  小鼠单克隆抗体[LN43] to Lamin B2

• 宿主

  Mouse

• 特异性

  This antibody reacts with an epitope located in the C-terminal part of lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. Please see the listed positive controls for this antibody. Other cell/tissue types have not been tested.

• 经测试应用

  适用于: Flow Cyt, IHC-Fr, WB, IPmore details

• 种属反应性

  与反应: Human, Zebrafish
  

• 免疫原

  Tissue, cells or virus corresponding to Lamin B2. Detergent insoluble fraction of potoroo cell line PtK1.
  Database link: Q03252

• 表位

  Epitope located in the C-terminal part of lamin B2.

• 阳性对照

• IHC-Fr: testicular germ cell tumour sections (see Machiels et al 1997), parabasal cells of skin epidermis (see Broers et al). WB: NCI-H125 nuclear cell lysate Jurkat cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: 99% PBS

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  LN43

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8983于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 84823 Human

• Omim: 150341 Human

• SwissProt: Q03252 Human

• Unigene: 728836 Human

• 别名

• LMN 2 antibody

• LMN B2 antibody

• LMN2 antibody

• LMNB 2 antibody

• LMNB2 antibody

• LMNB2_HUMAN antibody

• MGC2721 antibody

• RGD1563803 antibody

• LAMB 2 antibody

• LAMB2 antibody

• Lamin-B2 antibody

• 

  Western blot - Anti-Lamin B2 antibody [LN43] (ab8983)

  All lanes : Anti-Lamin B2 antibody [LN43] (ab8983) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
  Lane 4 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Observed band size: 75 kDawhy is the actual band size different from the predicted?
  Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 20 minutes
  

• 

  Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)

  ab8983 immunohistochemistry on a frozen section of human kidney showing nuclear lamina staining in the ductal epithelium.

• 

  Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)

  IHC-Fr of human kidney showing nuclear lamina staining in the ductal epithelium.  

• 

  Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (ab8983)

  Lamin B2 - Nuclear Envelope Marker was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Lamin B2 - Nuclear Envelope Marker (ab8983) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8983.
  Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
  Band: 73kDa: Lamin B2

• 

  Flow Cytometry - Anti-Lamin B2 antibody [LN43] (ab8983)

  Overlay histogram showing HeLA cells stained with ab8983 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8983, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)

  IF staining of a 9 days old zebrafish embryo.