Anti-Lamin A antibody [133A2]

• 产品名称

  Anti-Lamin A抗体[133A2]
  参阅全部 Lamin A 一抗

• 描述

  小鼠单克隆抗体[133A2] to Lamin A

• 宿主

  Mouse

• 特异性

  Reacts against lamin A exclusively (the antibody was raised against the carboxy-terminus of 98 amino acids present in lamin A and absent from lamin C. As a result, this antibody recognizes lamin A but not lamin C; See Machiels et al 1997). Lamins do not appear to be universally distributed among different cell and tissue types. ab8980 has been tested in testis parenchyma and testicular germ cell tumours (See Machiels et al 1997). Other cell/tissue types have not been tested.

• 经测试应用

  适用于: Flow Cyt, IHC-Fr, WB, IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant fragment corresponding to Human Lamin A (C terminal).
  Database link: P02545

• 表位

  Recognizes an epitope located between residues 598-611 of lamin A.

• 阳性对照

• Mouse heart normal tissue lysate - total protein (ab30291) can be used as a positive control in WB. This antibody gave a positive signal in the following cell types: HeLa WB: Wild-type HAP1 whole cell lysate; HeLa whole cell lysate

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: 99% PBS
  
  This IgG3 antibody has to be purified from the culture medium by ion-exchange chromatography, therefore it will contain remnants of bovine serum albumin.

• 纯度

  Ion Exchange Chromatography

• 克隆

  单克隆

• 克隆编号

  133A2

• 骨髓瘤

  P3x63-Ag8.653

• 同种型

  IgG3

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab8980于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4000 Human

• Entrez Gene: 16905 Mouse

• Omim: 150330 Human

• SwissProt: P02545 Human

• SwissProt: P48678 Mouse

• Unigene: 594444 Human

• Unigene: 243014 Mouse

• Unigene: 471227 Mouse

• 别名

• FPL antibody

• FPLD antibody

• HGPS antibody

• IDC antibody

• LAMIN A antibody

• lamin A/C antibody

• LAMIN C antibody

• Lamin-A/C antibody

• LDP1 antibody

• LFP antibody

• LMN 1 antibody

• LMN A antibody

• LMN C antibody

• LMNA antibody

• LMNA_HUMAN antibody

• LMNC antibody

• PRO1 antibody

• Renal carcinoma antigen NY-REN-32 antibody

• 70 kDa lamin antibody

• CDDC antibody

• EMD2 antibody

• 

  Western blot - Anti-Lamin A antibody [133A2] (ab8980)

  All lanes : Anti-Lamin A antibody [133A2] (ab8980) at 1/100 dilution
  
  Lane 1 : Wild-type HAP1 whole cell lysate
  Lane 2 : LMNA knockout HAP1 whole cell lysate
  Lane 3 : HeLa whole cell lysate
  Lane 4 : HepG2 whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Predicted band size: 74 kDa
  Observed band size: 74 kDa
  
  
  
  

  Lanes 1 - 4: Merged signal (red and green). Green - ab8980 observed at 74 kDa. Red - loading control, ab181602, observed at 37 kDa.

  ab8980 was shown to recognize  in wild-type HAP1 cells as signal was lost at the expected MW in LMNA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LMNA knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3pc Milk. Ab8980 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (ab8980)

  ICC/IF image of ab8980 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8980 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody [133A2] (ab8980)This image is courtesy of an Abreview submitted by Mr Carl Hobbs

  ab8980 staining of Lamin A in adult mouse liver sections. Formaldehyde-fixed paraffin-embedded sections of mouse liver tissue were incubated with ab8980 (1/2500) fo 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0 (ab64214). Please see accompanying abreview for additional information.

  See Abreview

• 

  Flow Cytometry - Anti-Lamin A antibody [133A2] (ab8980)

  Overlay histogram showing HeLa cells stained with ab8980 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8980, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (ab8980)

  These images show HeLa cells stained with Laminin A antibody - ab8980 (green) and with DAPI (blue). The pictures were kindly supplied as part of the review submitted by Dr Josef Gotzmann at Medical University of Vienna, Austria.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody [133A2] (ab8980)

  Immunohistochemistry on paraffin section of human colon.

• 

  Immunohistochemistry (Frozen sections) - Anti-Lamin A antibody [133A2] (ab8980)

  Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (ab8980)

  Immunocytochemical staining of fiboblasts showing nuclear lamina